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Series GSE67925 Query DataSets for GSE67925
Status Public on Apr 16, 2015
Title Gene transcriptional levels with or without acid treatment in Salmonella Typhimurium 14028s
Organism Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
Experiment type Expression profiling by high throughput sequencing
Summary Acetylation of lysine residues is conserved across organisms and plays important roles in various cellular functions. Maintaining intracellular pH homeostasis is crucial for the survival of enteric bacteria in acidic gastric tract. However, it remains unkown whether bacteria can utilize reversible protein acetylation system to adapt to acidic environment. Here we demonstrate that the protein acetylation/deacetylation is critical for Salmonella Typhimurium to survive in acidic environment.
We first used RNA-seq to analyze the transcriptome patterns under acid stress, and found that the transcriptional levels of genes involved in NAD+/NADH metabolism were significantly changed, leading to the increase of intracellular NAD+/NADH ratio. Moreover, acid stress down-regulated the transcriptional level of pat (encoding an acetyltranseferase) and genes encoding adenylate cyclase and cAMP-regulatory protein (CRP) which regulates pat positively. Acid signal also affects TCA cycle to promote the consumption of Ac-CoA, which reduced the donor of acetylation.
Lowered acetylation level is not only bacterial’s response to acid stress, but also positively regulates the survival rate of S. Typhimurium. The deletion mutant of pat had more stable intracellular pH, which paralleled with higher survival rate after acid treatment compared with the wild type strain and deletion mutant of cobB.
Our data suggest that bacteria can down-regulate protein acetylation level to prevent intracellular pH further falling in acid stress, and this work may provide a new perspective to understand the bacterial acid resistance mechanism.
 
Overall design To use RNA-seq to analyze the transcriptome patterns under acid stress
 
Contributor(s) Ren J, Sang Y, Lu J, Zhao M, Yao Y
Citation(s) 26070677
Submission date Apr 15, 2015
Last update date May 15, 2019
Contact name Yu Sang
E-mail(s) sshaobo2008@126.com
Phone +86 021 63846590 776534
Organization name Shanghai Jiao Tong University School of Medicine
Department Microbiology and Immunology
Street address 280 S. Chongqing Rd
City Shanghai
ZIP/Postal code 200025
Country China
 
Platforms (1)
GPL20046 Illumina HiSeq 2000 (Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S)
Samples (2)
GSM1658561 1 acid
GSM1658562 2 ck
Relations
SRA SRP057195
BioProject PRJNA281469

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Supplementary file Size Download File type/resource
GSE67925_RAW.tar 299.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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