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Series GSE71537 Query DataSets for GSE71537
Status Public on Jul 31, 2015
Title Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain [miRNA 3.0]
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary We performed a global analysis of extracellular miRNAs in the serum of human subjects diagnosed with Alcohol Use Disorder (AUD) to identify robust biomarkers of early brain damage or dysfunction. This was performed in a set of 20 AUD subjects and 10 age-matched controls. They were subjected to comprehensive medical, neuropsychological and neuroimaging tests, followed by comparison of miRNA levels found in peripheral blood serum. The levels of miRNAs were quantified using two independent high-throughput methods: Affymetrix microarray and Illumina next-generation RNA-sequencing. Cross-species validation was performed using rat drinking models and mouse neural stem cell culture models, with tissue specificity of the serum miRNAs examined using additional RNA-sequencing of serum and body tissues in untreated rats. This data series includes the human miRNA 3.0 GeneChip data only but is part of a larger superseries.
 
Overall design A total of 30 miRNA 3.0 GeneChips and 27 RNA-sequencing libraries were run on the same RNA samples from human subjects.
 
Contributor(s) Middleton FA, Ignacio C, Hicks SD, Burke P, Lewis L, Szombathyne-Meszaros Z
Citation(s) 26341662
Submission date Jul 30, 2015
Last update date Jul 27, 2018
Contact name Frank A Middleton
E-mail(s) middletf@upstate.edu
Phone 315-464-7721
Organization name SUNY Upstate Medical Univ
Department Neuroscience and Physiology
Street address 505 Irving Avenue
City Syracuse
State/province NY
ZIP/Postal code 13210
Country USA
 
Platforms (1)
GPL16384 [miRNA-3] Affymetrix Multispecies miRNA-3 Array
Samples (30)
GSM1836645 serum miRNA from alcohol use disorder subject replicate 1
GSM1836646 serum miRNA from alcohol use disorder subject replicate 2
GSM1836647 serum miRNA from alcohol use disorder subject replicate 3
This SubSeries is part of SuperSeries:
GSE71579 Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain
Relations
BioProject PRJNA291478

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71537_RAW.tar 42.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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