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| Status |
Public on Dec 01, 2007 |
| Title |
Confluent versus Sub-confluent HUVEC |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
HUVEC (N=3 isolates) were separately grown to sub-confluency, and to confluency. In order to study the effects of growth and contact inhibition on the transcriptome, the microarray gene expression profiles of these sub-confluent and confluent HUVEC were compared.
Keywords: HUVEC, confluency, contact inhibition
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| Overall design |
N=3 biological replicates, separately grown to sub-confluency, and concluency, respectively. Total RNA molecules were then isolated, and linearly amplified (1 round) according to the Eberwine method. After labeling the amplified cRNA molecules of these 6 samples (N=3 sub-confluent, N=3 confluent) with Cy5 Dyes, these were separately hybridized against Cy3-labeled common reference RNA molecules on glass-based 19K oligonucleotide microarray chips.
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| Contributor(s) |
Fontijn RD, Volger OL, Fledderus JO, Horrevoets AJ |
| Citation(s) |
18065521 |
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| Submission date |
Oct 16, 2007 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Oscar Leonard Volger |
| E-mail(s) |
Stephan.schirmer@uks.eu, a.m.vanderLaan@amc.uva.nl
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| Organization name |
Academic Medical Center
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| Department |
Cardiology
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| Street address |
Meibergdreef 15
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| City |
Amsterdam |
| State/province |
Noor Holland |
| ZIP/Postal code |
1105 AZ |
| Country |
Netherlands |
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| Platforms (1) |
| GPL4868 |
AMC-MAD Homo sapiens 19k ver 1.0 |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA103013 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9334_RAW.tar |
2.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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