|
| Status |
Public on May 12, 2015 |
| Title |
Salv CKO 1 H001-3100506351 |
| Sample type |
RNA |
| |
|
| Source name |
murine embryonic heart tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57bl/6 X 129 hybrid
genotype/variation: Salvador mutant (Salv CKO)
developmental stage: P8
tissue: whole heart
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Dissected P8 hearts were placed in RNAlater for tissue stabilization. Total RNA was then extracted using the RNeasy Micro Kit (Qiagen) and optical density was measured by NanoDrop ND-1000, Agilent RNA 6000 Nano Assay and agarose electrophoresis.
|
| Label |
Cy5
|
| Label protocol |
AminoAllyl aRNA labeling. The reactive amino group of 5-(3-aminoallyl)-UTP/5-(3-aminoallyl)-dUTP was used to conjugate the purified aRNA with the NHS-CyDye.
|
| |
|
| Hybridization protocol |
Phalanx Hybridization Protocol (http://www.phalanxbiotech.com/)
|
| Scan protocol |
Scanned by Phalanx Biotech Group (http://www.phalanxbiotech.com/)
|
| Data processing |
The raw data were re-scaled to account for the differences in individual hybridization intensities. We employed a rank-invariant scaling method to select genes (Tseng et al., 2001), which were then used for fitting of a non-linear normalization curve. Data was normalized using R Bioconductor software package (v.2.15.2).
|
| |
|
| Submission date |
Feb 06, 2013 |
| Last update date |
May 12, 2015 |
| Contact name |
Todd Ryan Heallen |
| E-mail(s) |
heallen@bcm.edu
|
| Phone |
7137985890
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular Physiology and Biophysics
|
| Lab |
Martin
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL13692 |
| Series (2) |
| GSE44103 |
Cardiac gene expression profiling of neonatal Salvador mutant mice |
| GSE138062 |
Actin cytoskeletal remodeling with protrusion formation is essential for heart regeneration in Hippo-deficient mice |
|
