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Status |
Public on Jul 31, 2006 |
Title |
Daudi_AICAR_7h_rep2 |
Sample type |
RNA |
|
|
Source name |
Daudi cells
|
Organism |
Homo sapiens |
Characteristics |
Burkitt's lymphoma 16-year-old Black male
|
Treatment protocol |
Daudi cells were cultured in the presence of 2.5 mM AICAR for 7 hr. After wash with cold PBS twice, cells were lysed in RLT buffer. Total RNA was extracted using an RNeasy
|
Growth protocol |
Daudi cells were cutured in RPMI1640 with 10% FBS. Cells were trated with 2.5 mM AICAR for 7 hr.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy kit
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with CodeLink Expression Assay Kit according to the standard manufacture's protocol from 2 microg total RNA
|
|
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Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 18 hr at 37C on CodeLink Human Whole Genome Bioarrays. Bioarrayss were washed and stained with Cy-5 Streptavidin
|
Scan protocol |
Bioarrays were scanned using arrayWoRx scanner.
|
Description |
none
|
Data processing |
CodeLink Expression Analysis software was used for primary data (raw data) extraction from bioarray images. Multiple bioarray data were normalized by mean using Spotfire.
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|
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Submission date |
May 11, 2006 |
Last update date |
May 11, 2006 |
Contact name |
Hiroshi Fukasawa |
E-mail(s) |
fukasawa@immd.co.jp
|
Organization name |
Institute of Medicinal Molecular Design, Inc.
|
Street address |
Kadokawa Hongo Bldg. 4F, 5-24-5 Hongo
|
City |
Bunkyo-ku, Tokyo |
ZIP/Postal code |
113-0033 |
Country |
Japan |
|
|
Platform ID |
GPL2895 |
Series (1) |
GSE4812 |
Expression data from Daudi cells stimulated with anti-CD40 and/or IL-4 for 6 hr with or without AICAR-pretreatment |
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