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Status |
Public on Nov 24, 2013 |
Title |
rat bladder, no obstruction_1A |
Sample type |
RNA |
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|
Source name |
urinary bladder, no obstruction
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Sprague-Dawley protocol: sham-operated time: control tissue: urinary bladder
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from urianry bladder tissue was prepared using the miRNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's recommendations
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
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Description |
1_Sham op 1A miRNA profiling
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Data processing |
The data were analyzed using expression console software version 1.1.2 using default analysis settings and RMA as normalization method.
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|
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Submission date |
May 19, 2013 |
Last update date |
Nov 24, 2013 |
Contact name |
Mari Ekman |
E-mail(s) |
mari.ekman@med.lu.se
|
Organization name |
Lund University
|
Street address |
BMC D12
|
City |
Lund |
State/province |
Sverige |
ZIP/Postal code |
221 84 |
Country |
Sweden |
|
|
Platform ID |
GPL16384 |
Series (1) |
GSE47080 |
Partial urethra obstruction in rat |
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