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Sample GSM1144515 Query DataSets for GSM1144515
Status Public on Nov 24, 2013
Title rat bladder, no obstruction_1A
Sample type RNA
 
Source name urinary bladder, no obstruction
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
protocol: sham-operated
time: control
tissue: urinary bladder
Extracted molecule total RNA
Extraction protocol Total RNA from urianry bladder tissue was prepared using the miRNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's recommendations
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
Description 1_Sham op 1A
miRNA profiling
Data processing The data were analyzed using expression console software version 1.1.2 using default analysis settings and RMA as normalization method.
 
Submission date May 19, 2013
Last update date Nov 24, 2013
Contact name Mari Ekman
E-mail(s) mari.ekman@med.lu.se
Organization name Lund University
Street address BMC D12
City Lund
State/province Sverige
ZIP/Postal code 221 84
Country Sweden
 
Platform ID GPL16384
Series (1)
GSE47080 Partial urethra obstruction in rat

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
api-bantam_st 0.08666259
api-let-7_st 6.764238
api-miR-1000_st -0.02981544
api-miR-100_st 10.15823
api-miR-10_st 9.787513
api-miR-124_st 0.794733
api-miR-137_st 0.1136097
api-miR-13a_st 0.114189
api-miR-14_st -0.0287087
api-miR-184a_st 2.608522
api-miR-184b_st 1.554901
api-miR-190_st -0.1097978
api-miR-1923_st 0.1466981
api-miR-1_st 2.905256
api-miR-210_st 2.515015
api-miR-219_st 0.3741351
api-miR-252a_st 0.3312103
api-miR-252b_st 0.1817785
api-miR-263a_st -0.01454055
api-miR-263b_st 0.4402301

Total number of rows: 25015

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM1144515_1_Sham_op_1A.CEL.gz 821.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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