|
| Status |
Public on Jun 26, 2013 |
| Title |
knee articular cartilage at T4, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
knee articular cartilage in middle-aged
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
age: middle-aged
time point: T4 (6-month)
tissue: knee articular cartilage
|
| Treatment protocol |
After grinded under the ice, cartilage tissue were collected for further total RNA extraction.
|
| Growth protocol |
The cartilage that was harvested individually from the right knee joint were stored at liquid nitrogen temperatures at once with protected by Rnasin inhibitor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip miRNA 3.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
|
| Description |
Rattus norvegicus_T4-3
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100 (Affymetrix Expression Expression Console v.1.1).
|
| |
|
| Submission date |
Jun 25, 2013 |
| Last update date |
Jun 26, 2013 |
| Contact name |
Xiao Chun Wei |
| E-mail(s) |
weixc666@163.com
|
| Phone |
86-0351-3365579
|
| Organization name |
the Second Hospital of Shanxi Medical University
|
| Department |
Department of Orthopaedics
|
| Lab |
Shanxi Key Laboratory of Bone and Soft Tissue Injury Repair
|
| Street address |
No.382, Wuyi Road,Taiyuan City.
|
| City |
Taiyuan City |
| State/province |
Shanxi Shanxi |
| ZIP/Postal code |
030001 |
| Country |
China |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE48266 |
Expression data from the rat knee articular cartilage at different developmental stages |
|
