|
Status |
Public on Nov 24, 2014 |
Title |
female sod155-/- s.c. |
Sample type |
RNA |
|
|
Source name |
Mouse -B6/SJL-mSOD1G93A-miR-155-/- (female)
|
Organism |
Mus musculus |
Characteristics |
cell type: Spinal cord FACS sorted adult FCRLS+ microglia strain/genotype: B6SJL-Tg(SOD1-G93A)1Gur/J / miR-155-/- gender: female
|
Treatment protocol |
NA
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using mirVanaTM miRNA isolation kit (Ambion) according to the manufacturer’s protocol
|
Label |
NA
|
Label protocol |
MG400 custom Nanostring codesets
|
|
|
Hybridization protocol |
according to the manufacturer’s protocol
|
Scan protocol |
according to the manufacturer’s protocol
|
Data processing |
Data was proccessed using nSolver, normalized to Positive controls, Housekeeping genes and negative controls
|
|
|
Submission date |
Nov 27, 2013 |
Last update date |
Nov 24, 2014 |
Contact name |
Oleg Butovsky |
E-mail(s) |
obutovsky@rics.bwh.harvard.edu
|
Phone |
1-617-525-5313
|
Organization name |
Brigham and Women's Hospital, Harvard Medical School
|
Department |
Center of Neurologic Diseases
|
Lab |
Dr. Oleg Butovsky
|
Street address |
77 Avenue Louis Pasteur, Office 614
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL18002 |
Series (2) |
GSE52803 |
MG400 profile of microglia from SOD miR155-KO mice |
GSE52947 |
Targeting miR-155 restores abnormal microglia and attenuates disease in SOD1 mice |
|