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Status |
Public on Jan 15, 2015 |
Title |
post-Tx biopsy 09, biopsy pair 1084-09, AKI group |
Sample type |
RNA |
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Source name |
kidney allograft biopsy
|
Organism |
Homo sapiens |
Characteristics |
tissue: kidney biopsy type: post-TX biopsy, acute tubular necrosis (ATN)
|
Extracted molecule |
total RNA |
Extraction protocol |
Kidney tissue in RNAlater, stored at -80°C. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Total RNA (1µg per sample) was labeled using the Affymetrix® FlashTag™ Biotin HSR RNA Labeling Kit and hybridized to the arrays as described by the manufacturer (Affymetrix, Santa Clara, CA).
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|
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Hybridization protocol |
The complementary RNA hybridization cocktail was incubated overnight at 48°C while rotating in a hybridization oven. After 16 hours of hybridization, the cocktail was removed and the arrays were washed and stained in an Affymetrix GeneChip fluidics station 450.
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Scan protocol |
Arrays were scanned on an Affymetrix GeneChip scanner 7G.
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Description |
microRNA expression data from post-TX biopsy, acute tubular necrosis (ATN)
|
Data processing |
Normalized expression signals were calculated from Affymetrix CEL files using RMA.
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|
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Submission date |
Jan 02, 2014 |
Last update date |
Jan 15, 2015 |
Contact name |
Julia Wilflingseder |
E-mail(s) |
julia.wilflingseder@meduniwien.ac.at
|
Organization name |
Medical University of Vienna
|
Department |
Nephrology and Dialysis
|
Lab |
Laboratory Rainer Oberbauer
|
Street address |
Währinger Gürtel 18-20
|
City |
Vienna |
ZIP/Postal code |
1090 |
Country |
Austria |
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|
Platform ID |
GPL16384 |
Series (2) |
GSE53771 |
Molecular regulation of acute kidney injury (miRNA) |
GSE53773 |
Molecular regulation of acute kidney injury |
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