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Sample GSM1300464 Query DataSets for GSM1300464
Status Public on Jan 15, 2015
Title post-Tx biopsy 09, biopsy pair 1084-09, AKI group
Sample type RNA
 
Source name kidney allograft biopsy
Organism Homo sapiens
Characteristics tissue: kidney
biopsy type: post-TX biopsy, acute tubular necrosis (ATN)
Extracted molecule total RNA
Extraction protocol Kidney tissue in RNAlater, stored at -80°C. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA (1µg per sample) was labeled using the Affymetrix® FlashTag™ Biotin HSR RNA Labeling Kit and hybridized to the arrays as described by the manufacturer (Affymetrix, Santa Clara, CA).
 
Hybridization protocol The complementary RNA hybridization cocktail was incubated overnight at 48°C while rotating in a hybridization oven. After 16 hours of hybridization, the cocktail was removed and the arrays were washed and stained in an Affymetrix GeneChip fluidics station 450.
Scan protocol Arrays were scanned on an Affymetrix GeneChip scanner 7G.
Description microRNA expression data from post-TX biopsy, acute tubular necrosis (ATN)
Data processing Normalized expression signals were calculated from Affymetrix CEL files using RMA.
 
Submission date Jan 02, 2014
Last update date Jan 15, 2015
Contact name Julia Wilflingseder
E-mail(s) julia.wilflingseder@meduniwien.ac.at
Organization name Medical University of Vienna
Department Nephrology and Dialysis
Lab Laboratory Rainer Oberbauer
Street address Währinger Gürtel 18-20
City Vienna
ZIP/Postal code 1090
Country Austria
 
Platform ID GPL16384
Series (2)
GSE53771 Molecular regulation of acute kidney injury (miRNA)
GSE53773 Molecular regulation of acute kidney injury

Data table header descriptions
ID_REF
VALUE log2 intensity (RMA)

Data table
ID_REF VALUE
14q0_st 5.191108
14qI-1_st 1.250035
14qI-1_x_st 2.184263
14qI-2_st 1.314709
14qI-3_x_st 2.828584
14qI-4_st 3.995381
14qI-4_x_st 3.543567
14qI-5_st 2.668833
14qI-6_st 2.326863
14qI-6_x_st 2.693335
14qI-7_st 5.452883
14qI-8_st 1.431965
14qI-8_x_st 1.730663
14qI-9_x_st 3.3602
14qII-10_st 1.493339
14qII-10_x_st 1.079401
14qII-11_st 2.069088
14qII-11_x_st 1.555177
14qII-12_st 5.048453
14qII-12_x_st 6.252862

Total number of rows: 25015

Table truncated, full table size 634 Kbytes.




Supplementary file Size Download File type/resource
GSM1300464_09_ATN_1084_miRNA.CEL.gz 868.3 Kb (ftp)(http) CEL
Processed data included within Sample table

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