|
| Status |
Public on Sep 05, 2014 |
| Title |
non receptive endometrium at day 3 of estrous cycle after in vitro embryo produce transfer pool2 |
| Sample type |
RNA |
| |
|
| Source name |
endometrium
|
| Organism |
Bos taurus |
| Characteristics |
breed: Simmental
gender: heifers
time: day 3
type of transfer: in vitro
receptive: no
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRI Reagent (Sigma, Taufkirchen, Germany) according to the manufacturer’s protocol. After DNaseI treatment the RNA was cleaned up using the RNeasy Kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
|
| Description |
miRNA expression data from endometrium
|
| Data processing |
Data were analysed with Affymetrix GCOS 1.1.1 software using global scaling to a target signal of 500.
|
| |
|
| Submission date |
Mar 31, 2014 |
| Last update date |
Sep 06, 2014 |
| Contact name |
Klaus Wimmers |
| E-mail(s) |
s.wimmers@fbn-dummerstorf.de
|
| Phone |
0049 3820868700
|
| Organization name |
Leibniz Institute for Farm Animal Biology (FBN)
|
| Street address |
Wilhelm-Stahl-Allee 2
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE56368 |
miRNA profile of the bovine pretransfer endometrium based on pregnancy success after in vivo and in vitro produced embryos transfer |
|
