|
| Status |
Public on May 25, 2015 |
| Title |
GF1 |
| Sample type |
SRA |
| |
|
| Source name |
Brain
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Microglia
strain: C57BL/6 (germ-free)
age: 6-8 weeks
|
| Treatment protocol |
no prior treatment
|
| Growth protocol |
Mice were housed under SPF or germ-free condiotions. Animals were 6-8 weeks old.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Adult microglia were harvested using density gradient separation. CD11b+CD45lo microglial cell populations were FACS-sorted and collected directly in cell lysis buffer.
3' end mRNA libraries were prepared for sequencing using a method developed at Dr. Ido Amit's lab, Weizmann Institute of Science
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Description |
polyA+ RNA
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
alignment: tophat with deafult parameters
Expression table done using ESAT http://garberlab.umassmed.edu/software/esat/
Genome_build: mm9
Supplementary_files_format_and_content: tab delimited
|
| |
|
| Submission date |
Oct 24, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Ido Amit |
| E-mail(s) |
ido.amit@weizmann.ac.il
|
| Phone |
972-8-9343338
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Immunology
|
| Street address |
234 Herzl st.
|
| City |
Rehovot |
| ZIP/Postal code |
760001 |
| Country |
Israel |
| |
|
| Platform ID |
GPL18480 |
| Series (1) |
| GSE62710 |
Commensal microbiota constantly control maturation and function of microglia in the central nervous system |
|
| Relations |
| BioSample |
SAMN03143044 |
| SRA |
SRX743393 |
