|
Status |
Public on Mar 24, 2008 |
Title |
3T3L1_adipocyte_UCP3 |
Sample type |
RNA |
|
|
Source name |
RNA was collected from 3T3L1 adipocytes expressing Uncoupling Protein 1.
|
Organism |
Mus musculus |
Characteristics |
3T3-L1 adipocytes with control plasmid. 3T3-L1 adipocytes expressing uncoupling protein 1 (UCP1)
|
Biomaterial provider |
Jayaraman Laboratory
|
Growth protocol |
3T3-L1 preadipocytes were grown until confluence and differentiated adipocytes using a standard adipogenic cocktail.
|
Extracted molecule |
total RNA |
Extraction protocol |
Adipocytes (10 days after differentiation) were used for extracting total RNA using the Nucleospin II RNA isolation kit (Clontech).
|
Label |
biotin
|
Label protocol |
Codelink Expression assay protocol.
|
|
|
Hybridization protocol |
Codelink Expression assay protocol.
|
Scan protocol |
Codelink Expression assay protocol.
|
Description |
RNA quality was assessed using the Agilent 2100 Bioanalyzer.
|
Data processing |
CodeLink Expression Analysis 4.1.0.29054
|
|
|
Submission date |
Jan 04, 2007 |
Last update date |
Mar 24, 2008 |
Contact name |
Arul Jayaraman |
Organization name |
Texas A&M University
|
Department |
Artie McFerrin Department of Chemical Engineering
|
Street address |
3122 TAMU
|
City |
College Station |
State/province |
TX |
ZIP/Postal code |
77843-3122 |
Country |
USA |
|
|
Platform ID |
GPL2897 |
Series (1) |
GSE6643 |
Effect of forced uncoupling protein-1 expression on 3T3-L1 adipocyte gene expression |
|