|
| Status |
Public on Dec 01, 2015 |
| Title |
EC-70124 500nM 24h rep1 |
| Sample type |
RNA |
| |
|
| Source name |
TNBC cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
treatment: EC-70124 500nM 24h
|
| Treatment protocol |
cells were treated with 500 nM EC-70124 for 24h
|
| Growth protocol |
MDA-MB-231 cells were grown in DMEM with 10% of FBS and added to a final concentration of 500.000 cells/plate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and purified using the Rneasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
cRNA was labelled using the BioArray RNA labeling kit (Enzo Life Sciences, Farmingdale, NY
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol
|
| Scan protocol |
Total RNA was extracted from purified cell populations using RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). Labelling and hybridizations were performed according to protocols from Affymetrix. Briefly, 100 ng of total RNA were amplified and labeled using the WT Plus reagent kit (Affymetrix) and then hybridized to Human Gene 2.0 ST Array (Affymetrix). Washing and scanning were performed using GeneChip System of Affymetrix (GeneChip Hybridization Oven 645, GeneChip Fluidics Station 450 and GeneChip Scanner 7G).
|
| Data processing |
The data were processed using the dChip software (Dana Farber Cancer Institute, Boston, MA)
|
| |
|
| Submission date |
Jun 22, 2015 |
| Last update date |
Dec 01, 2015 |
| Contact name |
Francisco Moris |
| E-mail(s) |
fmv@entrechem.com
|
| Organization name |
EntreChem SL
|
| Street address |
Edificio Cientifico Tencológico, Campus El Cristo
|
| City |
Oviedo |
| ZIP/Postal code |
33006 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE70115 |
Antitumor activity of the novel multi-kinase inhibitor EC-70124 in triple negative breast cancer |
|
