|
| Status |
Public on Mar 31, 2016 |
| Title |
PA_GW_UlkKD_2 |
| Sample type |
RNA |
| |
|
| Source name |
AML-12 cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
treatment: Palmitic acid and GW3965 and Ulk1 siRNA
|
| Treatment protocol |
AML-12 cells co-treated with 0.75mM PA+/- 10µM GW3965 for 24 h +/- Ulk1 SiRNA
|
| Growth protocol |
Aml-12 mose hepatocytes were culture in 1:1 DMEM/F12 Media with 10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using InviTrap Spin Cell RNA Mini Kit (Stratec) according to the manufacturer's instructions. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
Data was quantile normalized in Partek Genomics Suite software (version 6.6)
|
| |
|
| Submission date |
Oct 15, 2015 |
| Last update date |
Mar 31, 2016 |
| Contact name |
Paul Michael Yen |
| E-mail(s) |
paul.yen@duke-nus.edu.sg
|
| Organization name |
Duke-NUS Graduate Medical School Singapore
|
| Department |
Cardiovascular & Metabolic disorder Program
|
| Lab |
Laboratory of Hormonal Regulation
|
| Street address |
8 College Road
|
| City |
Singapore |
| ZIP/Postal code |
169857 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL18752 |
| Series (1) |
| GSE74059 |
Transcriptional regulation of genes in AML12 cells treated with Palmitic acid, LXR lingand (GW3965) and Ulk1 siRNA |
|
