|
| Status |
Public on Nov 03, 2015 |
| Title |
Leucocytes_MS patient in relapse_rep24 |
| Sample type |
RNA |
| |
|
| Source name |
MS relapse
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: leucocytes
disease state: MS relapse
gender: male
age: 39
|
| Extracted molecule |
total RNA |
| Extraction protocol |
LeucoLOCK Total RNA isolation system with the alternative protocol to recover small RNA fraction (ThermoFisher)
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled using FlashTag Biotin HSR RNA labeling kit (Genisphere)
|
| |
|
| Hybridization protocol |
GeneChip Hybridization, Wash and Stain kit was used for hybridization in a GeneChip Hybridization oven 640 and in a GeneChip Fluidics Station 450 using the protocol FS450-0003, as explained in the protocol provided by Affymetrix.
|
| Scan protocol |
Arrays were scanned in a GeneChip 7G scanner and analysed in Affymetrix GeneChip Command Console Scan Control v4.0.0.1567.
|
| Description |
mir99-276BV
|
| Data processing |
Quality control, summarization and normalization of the data were performed with miRNA QC Tool v.1.0.33.0 following the following workflow: Background detection, RMA Global Background correction, Quantile normalization and summarization with median polish.
|
| |
|
| Submission date |
Nov 02, 2015 |
| Last update date |
Nov 03, 2015 |
| Contact name |
Maider Muñoz-Culla |
| E-mail(s) |
maider.munoz@ehu.eus
|
| Organization name |
Biodonostia Institute
|
| Street address |
Paseo Dr.Begiristain s/n
|
| City |
San Sebastian |
| ZIP/Postal code |
20014 |
| Country |
Spain |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE74579 |
Small nucleolar RNA and microRNA expression data from multiple sclerosis patients in relapse and remission. |
| GSE74586 |
SncRNA (microRNA & snoRNA) expression in leucocytes from multiple sclerosis relapse and remission and cultured peripheral blood mononuclear cells |
|
