|
| Status |
Public on Nov 01, 2016 |
| Title |
A2780_CP20_Exosome, Biological rep. 1 |
| Sample type |
RNA |
| |
|
| Source name |
A2780-CP20 Exosome
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A2780-CP20
tissue type: Ovarian cancer
|
| Treatment protocol |
N/A
|
| Growth protocol |
All cells were maintained at 37°C with 5% CO2 and 95% air.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ovarian cancer cells were plated in cell culture medium containing 10% FBS and cultured for 24 h to 70% confluence. Media were replaced with 10% exosome-depleted FBS and cells were grown for 24hours more. Cells were collected by centrifugation and exosomes were isolated using supernatant with Total exosome isolation reagent (Invitrogen, Waltham, MA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated polyA tailed RNA were prepared according to the standard Affymetrix protocol (FlashTag Biotin HSR RNA Labeling Kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Following biotinylation, hybridization coctailed was prepared with 21.5ul labeled sample and incubated at 95C for minutes , then 45C for 5minutes. Following sample loading, arrays were loaded into trays and incibated at 48C abd 60rpm for 16 to 18hours.
|
| Scan protocol |
The instructions for using the scanner can be found in Affymetrix Command Console User Guide.
|
| Description |
Epithelial
miRNA
|
| Data processing |
The data were analyzed with Expression Console. RMA + DABG analysis was performed for the normalization.
|
| |
|
| Submission date |
Dec 31, 2015 |
| Last update date |
Nov 01, 2016 |
| Contact name |
Gabriel Lopez-Berestein |
| E-mail(s) |
glopez@mdanderson.org
|
| Organization name |
MD Anderson Cancer Center
|
| Department |
Experimental Therapeutics
|
| Lab |
Lopez Lab
|
| Street address |
1901 East Rd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE76449 |
miRNA Expression Data from Ovarian Cancer Exosomes and Cells of Origin |
|
