|
Status |
Public on Jul 08, 2008 |
Title |
Lethal Yellow mice pioglitazone treated #4 |
Sample type |
RNA |
|
|
Source name |
ovary
|
Organism |
Mus musculus |
Characteristics |
Ovary from pioglitazone-treated obese Lethal Yellow female mouse (C57BL/6J Ay/a), 180 days of age, treated by gavage with pioglitazone (0.1 mg/day) from 120 to 180 days of age.
|
Extracted molecule |
total RNA |
Extraction protocol |
The ovaries were placed in RNAlater (Ambion) in the surgical suite. The ovaries were homogenized in TRI reagent (MRC) using a Polytron (Brinkmann). The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity was evaluated with the RNA 6000 NanoLabChip in an Agilent Bioanalyzer.
|
Label |
Alexa-647
|
Label protocol |
see CodeLink Gene Expression System: Manual Labelled cRNA Target Preparation (0080074-00/Rev. AA/2004-04)
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|
|
Hybridization protocol |
CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection (080075-00/Rev. AA/2004-04)
|
Scan protocol |
Scanning CodeLink Bioarrays on Agilent G2565 microarray scanner (63-0050-80/Rev.AC/2004-08)
|
Description |
IACUC approval was obtained for all animal treatments.
|
Data processing |
global median normalization, CodeLink Expression Analysis 4.1.0.4163
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|
|
Submission date |
Aug 15, 2007 |
Last update date |
Feb 12, 2009 |
Contact name |
Kathleen M Eyster |
E-mail(s) |
Kathleen.Eyster@usd.edu
|
Organization name |
University of South Dakota
|
Department |
Basic Biomedical Sciences
|
Street address |
414 E. Clark St.
|
City |
Vermillion |
State/province |
SD |
ZIP/Postal code |
57069 |
Country |
USA |
|
|
Platform ID |
GPL2897 |
Series (1) |
GSE8806 |
Pioglitazone treatment of Lethal Yellow mice |
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