|
Status |
Public on Nov 16, 2016 |
Title |
SFC180-01-01 |
Sample type |
genomic |
|
|
Source name |
induced pluripotent stem cell line derived from dermal fibroblasts, control donor 180, clone 1
|
Organism |
Homo sapiens |
Characteristics |
cell type: induced pluripotent stem cell line derived from dermal fibroblasts genotype/variation: control gender: female
|
Treatment protocol |
untreated cell samples; Fibroblasts grown in ADMEM + 10% FCS; iPSc lines all grown in mTeSR-1 medium + ES-qualified matrigel prior to harvest
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted using QIAGEN DNeasy blood and tissue kit
|
Label |
Biotin (C, G), Dinitrophenyl (A, T)
|
Label protocol |
Standard Illumina labelling protocol
|
|
|
Hybridization protocol |
Standard Illumina hybridisation protocol
|
Scan protocol |
Standard Illumina scanning protocol for BeadChip Arrays
|
Description |
mTeSR-1 + matrigel single replicate humanomniexpress-24-v1-1-a
|
Data processing |
The scanned data files were processed by GenomeStudio Genotyping module v1.9, using the cluster file and manifest HumanCytoSNP-12v2-1_H provided by Illumina for GPL13829, and humanomniexpress_24v1-0_a or humanomniexpress-24-v1-1-a for GPL19699 as indicated (sheets GPL19699 1-0 and GPL19699 1-1 respectively). Raw data was also uploaded to KaryoStudio (Illumina) for generation of karyotype reports (see publication).
|
|
|
Submission date |
Nov 15, 2016 |
Last update date |
Nov 16, 2016 |
Contact name |
Sally A Cowley |
E-mail(s) |
sally.cowley@path.ox.ac.uk
|
Phone |
+44 (0) 1865 275600
|
Organization name |
University of Oxford
|
Department |
Sir William Dunn School of Pathology
|
Lab |
James Martin Stem Cell Facility
|
Street address |
South Parks Road
|
City |
Oxford |
ZIP/Postal code |
OX1 3RE |
Country |
United Kingdom |
|
|
Platform ID |
GPL22678 |
Series (2) |
GSE89878 |
Gene expression data from induced Pluripotent Stem cells from Parkinson's Disease patients harbouring amplification of, or mutations in the SNCA gene, and from healthy control donors [genotype] |
GSE89886 |
Gene expression data from induced Pluripotent Stem cells from Parkinson's Disease patients harbouring amplification of, or mutations in the SNCA gene, and from healthy control donors |
|