|
| Status |
Public on Aug 30, 2017 |
| Title |
TAC_DMSO_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
cardiomyocyte
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
protocol: TAC_DMSO
|
| Treatment protocol |
C57BL/6J mice subject to TAC (transverse aortic constriction) were treated with BIX-01294 for 1 week via subcutaneous mini-osmotic pumps. The control groups included cardiomyocyte-enriched populations isolated from DMSO-treated mice after TAC and from sham surgery mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 5
|
| Data processing |
The data exported from GenomeStudio V2011.1 were analysed with Lumi/Limma in R. Data preprocessing was performed using lumi package: - Background Correction: bgAdjust - Variance Stabilizing Transform method: vst - Normalization method: quantile The differentianly expressed genes were identified using limma package.
|
| |
|
| Submission date |
Jan 17, 2017 |
| Last update date |
Aug 30, 2017 |
| Contact name |
Simone Serio |
| E-mail(s) |
simone.serio@humanitasresearch.it
|
| Organization name |
Humanitas Research Hospital
|
| Department |
Cardiovascular Medicine
|
| Street address |
Via Alessandro Manzoni, 56
|
| City |
Rozzano |
| State/province |
Milano |
| ZIP/Postal code |
20089 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE93691 |
Gene expression profiling of cardiomyocyte-enriched populations isolated from mice subject to transverse aortic constriction (TAC) and treated with BIX-01294 for 1 week |
| GSE93754 |
The genomic distribution and gene expression profiling of cardiomyocyte-enriched populations |
|
