|
| Status |
Public on May 08, 2008 |
| Title |
GM8921A, unaffected brother |
| Sample type |
RNA |
| |
|
| Source name |
GM8921A
|
| Organism |
Homo sapiens |
| Characteristics |
unaffected brother
|
| Biomaterial provider |
Corielle
|
| Growth protocol |
B-Cells from Corielle were incubated at 37oC and 5% CO2 overnight, the day after cells are counted. Cells were expanded in RPMI 1640 with 2mM L-glutamine and 15% FBS following Corielle's lymphoblast line maintenance protocols. 1x10^7 cells were spun down and resuspended in 2 ml Tri-Reagent and frozen overnight at -80oC prior to RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Std Tri-Reagent protocol followed by cleanup on RNEasy columns using an on-column DNase step. RNA quality is checked using a Nanodrop and an Agilent Bioanalyzer
|
| Label |
biotin-11-UTP
|
| Label protocol |
Standard Codelink protocol
|
| |
|
| Hybridization protocol |
Standard Codelink protocol
|
| Scan protocol |
Standard Codelink protocol on Axon genePix 4000B
|
| Description |
GM8921A, unaffected brother, 46yrs, family 2108
|
| Data processing |
Codelink Expression Analysis Softwarev4.2
|
| |
|
| Submission date |
Jan 03, 2008 |
| Last update date |
May 08, 2008 |
| Contact name |
Sridar V Chittur |
| E-mail(s) |
schittur@albany.edu
|
| Phone |
518-591-7215
|
| Organization name |
SUNY-University at Albany
|
| Department |
Center for Functional Genomics
|
| Lab |
Microarray Core
|
| Street address |
One Discovery Drive, CRC 342G
|
| City |
Rensselaer |
| State/province |
NY |
| ZIP/Postal code |
12144 |
| Country |
USA |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE10064 |
Gene expression in immortalized B-lymphocytes derived from patients with multiple sclerosis |
|
