Mice were treated daily with Vaseline cream (mock) or IMQ on the shaved dorsal skin for 7 consecutive days. In vanillin groups, mice were applied topically with IMQ and given orally with 100 mg/kg vanillin.
Extracted molecule
total RNA
Extraction protocol
Total RNAs were extracted from muscle tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
Label
Cy5
Label protocol
Fluorescence-labeled RNA targets were prepared from 5 μg of total RNA using MessageAmpTM aRNA kit (Ambion, Austin, TX, USA) and Cy5 dye (Amersham Pharmacia, Piscataway, NJ, USA).
Hybridization protocol
Fluorescent targets were hybridized to Mouse Whole Genome OneArrayTM (Phalanx Biotech Group, Hsinchu, Taiwan).
Scan protocol
The slides were scanned by an Axon 4000 scanner (Molecular Devices, Sunnyvale, CA, USA).
Description
Total RNAs were extracted from muscle tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
Data processing
The Cy5 fluorescent intensity of each spot was analyzed by Genepix 4.1 software (Molecular Devices, Sunnyvale, CA, USA). The signal intensity of each spot was corrected by subtracting background signals in the surrounding. We filtered out spots that signal-to-noise ratio was less than 0 or control probes. Spots that pass aforementioned criteria were normalized by R program in the limma package.
