|
| Status |
Public on Sep 30, 2009 |
| Title |
PGP1TD |
| Sample type |
RNA |
| |
|
| Source name |
Human primary fibroblasts, PGP1
|
| Organism |
Homo sapiens |
| Characteristics |
Male human primary fibroblasts infected with adenoviral pluripotency factors
|
| Treatment protocol |
Adenoviral OCT4,SOX2,KLF4,MYC at 10 MOI for 7 days
|
| Growth protocol |
ES media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Per Qiagen RNeasy Mini Protocol
|
| Label |
Biotin
|
| Label protocol |
See manufacturer's protocol (http://www.illumina.com/downloads/WholeGenomeExpressionTechnicalBulletin.pdf)
|
| |
|
| Hybridization protocol |
See manufacturer's protocol (http://www.illumina.com/downloads/WholeGenomeExpressionTechnicalBulletin.pdf)
|
| Scan protocol |
See manufacturere's (Illumina,Inc) technical manual (BeadXpress Reader)
(http://www.illumina.com/downloads/BeadXpressReaderDataSheet.pdf)
|
| Description |
Data from UCSD Kun Zhang
|
| Data processing |
Quantile Normalization
BeadStudio (Illumina, Inc). For details, see http://www.illumina.com/downloads/GXBeadStudioNormalization_TechNote.pdf
|
| |
|
| Submission date |
Aug 18, 2008 |
| Last update date |
Oct 03, 2008 |
| Contact name |
Je Hyuk Lee |
| E-mail(s) |
jehyuklee@genetics.med.harvard.edu
|
| Phone |
(617) 432-7157
|
| Organization name |
Harvard Medical School
|
| Department |
Wyss Institute
|
| Lab |
Church
|
| Street address |
3 Blackfan Circle, CLSB 528
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE12478 |
Induction of developmental and inflammatory transcriptomes in PGP fibroblasts |
|
