|
| Status |
Public on Feb 27, 2009 |
| Title |
U937 cells_LPS_2hrs |
| Sample type |
RNA |
| |
|
| Source name |
U937 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U937 cells
developmental stage: Activated macropahges
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using QIAGEN RNeasy Plus Mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
cRNA synthesis and biotin-labeling was performed using Illumina TotalPrep RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicates averaged
|
| Data processing |
Raw data was acquired as signal intensity of each spot on the array, and signal values of technical and biological replicates were averaged. Normalization was done by variance stabilization method (Weber et al. 2002). Differentially expressed genes were determined based on a comparison of normalized signal intensity ratio between experimental (differentiated and/or stimulated U937 cells) and control (undifferentiated U937 cells) samples
|
| |
|
| Submission date |
Feb 27, 2009 |
| Last update date |
Feb 27, 2009 |
| Contact name |
Agnes Baek |
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Dept. of Vetebrate Genomics
|
| Street address |
Ihnestrasse 63-73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL5104 |
| Series (1) |
| GSE15038 |
Identification of novel transcriptional regulators involved in differentiation and function of monocyte and macropahge |
|
