|
| Status |
Public on Feb 28, 2010 |
| Title |
Mutant Histone H2B K111A replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Mutant yeast strain Histone H2B K111A
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: WY317 (mutant Histone H2B K111A)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were grown in YPD media until mid-log phase. Cells were harvested at OD600 of 0.35-0.7. Cells were lysed and RNA was extracted by hot acid phenol chloroform and phenol chloroform isoamyl alcohol extraction.
|
| Label |
biotin
|
| Label protocol |
Approximately 20 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Mutant yeast strain Histone H2B K111A
|
| Data processing |
Affymetrix GeneChip Operating Software v.1.4
|
| |
|
| Submission date |
Mar 12, 2009 |
| Last update date |
Mar 12, 2009 |
| Contact name |
John J. Wyrick |
| E-mail(s) |
jwyrick@vetmed.wsu.edu
|
| Phone |
509-335-8785
|
| Organization name |
Washington State University
|
| Department |
School of Molecular Biosciences
|
| Street address |
Biotechnology Life Sciences 241
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99164 |
| Country |
USA |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
|
