|
| Status |
Public on May 08, 2009 |
| Title |
mouse 3 with the human c-Src transgene inserted downstream of the mouse metallothionein promoter |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
genotype: human c-Src transgene inserted downstream of the mouse metallothionein promoter
tissue: liver
strain: c57bl/6
age: 14 month old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol reagent (Invitrogen) Cleanup with Qiagen Rneasy
|
| Label |
Alexa-647 Streptavidin
|
| Label protocol |
Biotin labeled cRNA was prepared by linear amplification of the poly(A)+ RNA population within the total RNA sample
|
| |
|
| Hybridization protocol |
Array hybridized at 37C for 18h
|
| Scan protocol |
Array scanned with Axon GenePixPro 4000B
|
| Description |
liver tissue from 14 month old C57Bl/6 mouse
|
| Data processing |
The output was generated for GeneSpring import by CodeLink Expression Analysis Software v4.1 (GE Healthcare), which creates a txt file compatible with GeneSpring. Raw values represent the final number of the spot after background is subtracted out. Normalized values represent median global normialzation method - raw intensity/median intensity of all spots on the array
|
| |
|
| Submission date |
Apr 23, 2009 |
| Last update date |
May 07, 2009 |
| Contact name |
Rosalyn B. Irby |
| E-mail(s) |
rirby@psu.edu
|
| Phone |
717-531-5035
|
| Fax |
717-531-0743
|
| URL |
http://www.psu.edu
|
| Organization name |
Pennsylvania State University Hershey Medical Center
|
| Department |
Medicine
|
| Street address |
500 University Drive
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platform ID |
GPL2897 |
| Series (1) |
| GSE15815 |
Src kinase Induces Tumor Formation in the cSRC C57BL/6 Mouse |
|
