|
| Status |
Public on Aug 31, 2021 |
| Title |
STZ4_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
STZ-induced DM in 8 week mouse, replicate 1, sample 1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
treatment: STZ
age: 8 weeks-old
|
| Treatment protocol |
We administrated the MENS treatments by printed circuit board empowered by MENS device for 1 h/day, 5 days/week with 6 weeks.
|
| Growth protocol |
The mice were used and growth for 8 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the liver tissues using TRIzol Reagent through chloroform/isopropanol purification. RNA quantity and purity was assessed using NanoDrop ND-1000. Pass criteria for absorbance ratios are established as A260/A280 ≥ 1.8 and A260/A230 ≥ 1.5. RIN values are assessed and examined using Agilent RNA 6000 Nano assay to determine RNA integrity.
|
| Label |
Cy5
|
| Label protocol |
Fluorescent aRNA targets were prepared from 1 μg total RNA samples using OneArray® Amino Allyl aRNA Amplification Kit (Phalanx Biotech Group, Taiwan) and Cy5 dye (GE Healthcare).
|
| |
|
| Hybridization protocol |
Total samples were hybridized using Phalanx OneArray Plus Protocol. Non-specific binding targets were washed away after 16 hours.
|
| Scan protocol |
The slides scanned using Agilent 0.1 XDR Protocol.
|
| Data processing |
GPR files were loaded into Rosetta Resolver® System (Rosetta Biosoftware) for data processing. The raw data normalized using the normalizeQuantiles function from 'limma' package (v. 3.30.7).
|
| |
|
| Submission date |
Aug 05, 2020 |
| Last update date |
Aug 31, 2021 |
| Contact name |
Yong Chun Luo |
| Organization name |
National Chiao Tung University
|
| Street address |
Boai Street
|
| City |
Hsinchu |
| ZIP/Postal code |
300 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL13692 |
| Series (1) |
|
