|
| Status |
Public on Sep 28, 2021 |
| Title |
day3-pregnant mouse mammary gland fibroblasts (non-cultured) replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
fibroblasts from mouse mammary glands, 3 days pregnancy, 12-13 weeks of age
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL6
Sex: female
age: 12-13 weeks
cell type: Fibroblasts
culturing method: non-cultured
condition: day3-pregnant
|
| Treatment protocol |
no treatment was applied
|
| Growth protocol |
Mice (strain C57/BL6) were kept in conventional M3 cages bedded with wood chips and paper nesting material in a temperature-controlled environment at 21±1°C, 45-55 % humidity on a 12-hr light/dark cycle. Food and water were provided ad libitum. Mice were allowed a 7-day acclimatisation period after arrival on site prior to experimental use. At 12 weeks of age, females were mated overnight and investigated for plug formation to indicate successful mating. Males were removed at this stage. In parallel, age-matched littermates were kept seperate. Plugged females were left for 72 hours after plug formation before sacrificing them using schedule 1 method. 2nd, 3rd, and 4th mammary glands were immediately removed and one flank used for fibroblast isolation. Fibroblasts were isolated as described by Ibrahim et al., 2017.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Direct-zol™ RNA MicroPrep (Zymo Research) for freshly isolated fibroblasts (500-2,000 cells) as per manufacturer’s instructions and further described by Ibrahim et al., 2017. RNA amplification was subsequently performed using a NuGen-RNA amplification kit according to the manufacturer's protocol with the advised alterations for use in Illumina Genebead Microarray hybridisation.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
9482914088_A
amplified total RNA
Preg 1
|
| Data processing |
The data were normalised using quantile normalisation. Processed data file includes p-values, median values and fold-change values.
|
| |
|
| Submission date |
Feb 24, 2021 |
| Last update date |
Sep 28, 2021 |
| Contact name |
Torsten Stein |
| E-mail(s) |
Torsten.Stein@glasgow.ac.uk
|
| Organization name |
University of Glasgow
|
| Department |
School of Medicine
|
| Lab |
Mammary Gland Morphogenesis and Breast Cancer
|
| Street address |
College of MVLS
|
| City |
Glasgow |
| ZIP/Postal code |
G12 8QQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE167438 |
Pregnancy-activated fibroblasts of the mouse mammary gland |
|
