|
| Status |
Public on Jul 11, 2011 |
| Title |
DHT for 16h, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-MB-453 cells treated with 10nM DHT for 16h
|
| Organism |
Homo sapiens |
| Characteristics |
agent: 5a-dihydrotestosterone
time point: 16h
cell line: MDA-MB-453
disease state: breast cancer
|
| Treatment protocol |
The MDA-MB-453 cells were grown in the phenol red-free medium supplemented with 5% charcoal/dextran-treated fetal bovine serum for 3 days, followed by treatment of vehicle or 10 nM DHT for time course.
|
| Growth protocol |
The MDA-MB-453 cells were maintained in DMEM supplemented with 10% fetal bovinw serum at 37oC incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
Gene expression data from 16h DHT treated MDA-MB-453 cells
|
| Data processing |
Raw gene expression values were obtained using Affymetrix GeneChip software and further analyzed using the Robust Multichip Average (RMA) algorithm and the MultiExperiment Viewer (MeV) software.
|
| |
|
| Submission date |
Apr 01, 2011 |
| Last update date |
Jul 11, 2011 |
| Contact name |
Yiwen Chen |
| E-mail(s) |
ychen26@mdanderson.org
|
| Organization name |
University of Texas MD Anderson Cancer Center
|
| Department |
Bioinformatics and Computational Biology
|
| Lab |
Yiwen Chen
|
| Street address |
1400 Pressler Street
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE28305 |
Effect of 5a-dihydrotestosterone on breast cancer cell line MDA-MB-453 |
| GSE28789 |
Breast cancer cell line MDA-MB-453 response to DHT |
|
