|
| Status |
Public on Apr 30, 2024 |
| Title |
Larval body wall muscles, madRNAi1 |
| Sample type |
SRA |
| |
|
| Source name |
Larval body wall musculature
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Larval body wall musculature
cell type: Muscle enriched
genotype: Dmef2-Gal4>UAS-madRNAi
treatment: Mad knockdown
|
| Treatment protocol |
Tissue-specific knockdown under Dmef2 promotor
|
| Growth protocol |
Larvae grown to wandering third instar stage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction
RNA libraries were prepared using Poly A enrichment following oligo-dT-mediated purification
PE50 run
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
FASTQ files were mapped to Drosophila genome using Rsubread in R
Count matrix was generated using the GenomicAlignments package
Assembly: dm6
Supplementary files format and content: comma-separated .csv table including normalized counts for each sample
|
| |
|
| Submission date |
Apr 10, 2024 |
| Last update date |
Apr 30, 2024 |
| Contact name |
Mary Baylies |
| E-mail(s) |
M-BAYLIES@SKI.MSKCC.ORG
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Street address |
430 E 67th St # 310
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065-6304 |
| Country |
USA |
| |
|
| Platform ID |
GPL21306 |
| Series (1) |
| GSE263700 |
BMP effector Mad knockdown in Drosophila musculature of wandering third instar larvae |
|
| Relations |
| BioSample |
SAMN40921077 |
| SRA |
SRX24216563 |
