|
| Status |
Public on Aug 09, 2024 |
| Title |
hypothalamus tissue, puberty, replicate3 |
| Sample type |
SRA |
| |
|
| Source name |
the hypothalamic tissue
|
| Organism |
Ovis aries |
| Characteristics |
tissue: the hypothalamic tissue
group: puberty
|
| Treatment protocol |
The hypothalamic tissue was placed in RNase-free cryotubes and frozen in liquid nitrogen.
|
| Growth protocol |
Hypothalamic tissue was collected from prepubertal, pubertal, and postpubertal sheep.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA content from the hypothalami was extracted using TRIzol reagent.
Clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v4-cBot-HS (Illumina) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Sheep_I813-T06
|
| Data processing |
In the present study, transcript libraries from Dolang sheep at the developmental stages of prepuberty, puberty, and postpuberty were constructed and assayed by high-throughput RNA-seq.
Clean reads were obtained using the program Trimmomatic v0.32 by removing reads containing adapters, reads containing poly-N, and low-quality raw reads.
Clean reads were then aligned and mapped to the sheep genome using HISAT2.
The matched reads were assembled and gene or transcript expression was calculated using StringTie.
Assembly: GCF_016772045.2
Supplementary files format and content: tab-delimited text file includes raw counts for each Sample.
Supplementary files format and content: tab-delimited text file includes raw counts for each Sample.
|
| |
|
| Submission date |
Aug 05, 2024 |
| Last update date |
Aug 13, 2024 |
| Contact name |
Rong Xuan |
| E-mail(s) |
xuanrongtarim@taru.edu.cn
|
| Phone |
86+18853857973
|
| Organization name |
College of Animal Science and Technology
|
| Department |
Tarim University
|
| Street address |
No. 1487, Tarim Avenue East, Alar City, Xinjiang
|
| City |
Alar |
| State/province |
XinJiang |
| ZIP/Postal code |
843300 |
| Country |
China |
| |
|
| Platform ID |
GPL15670 |
| Series (1) |
| GSE273981 |
Transcriptome sequencing of sheep hypothalamus tissue reveals the regulatory role of lncRNA in the mechanism of pubertal estrus initiation |
|
| Relations |
| SRA |
SRX12744825 |
| BioSample |
SAMN22513945 |
