|
| Status |
Public on Feb 13, 2013 |
| Title |
MM_#03R_Exon1.0ST |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
tumor: multiple myeloma
tissue: bone marrow
cell type: purified CD138+ plasma cells
sample type: relapse
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed according to the manufacturer's instructions using kit:
RLT+
|
| Label |
Biotin
|
| Label protocol |
According to manufacturer protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 µg of ST-cDNA were hybridized for 16-20 hours at 45°C on GeneChip Human Exon 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Myeloma
|
| Data processing |
Affymetrix Expression Console 1.1.2 using RMA Gene core analysis with default parameters.
|
| |
|
| Submission date |
Apr 19, 2012 |
| Last update date |
Feb 13, 2013 |
| Contact name |
Wilfried Gouraud |
| Organization name |
ICO - UMGC
|
| Department |
Integrated Center of Oncology René Gauducheau
|
| Lab |
Omics Data Science Unit
|
| Street address |
bd Jacques Monod
|
| City |
Saint Herblain |
| ZIP/Postal code |
44805 |
| Country |
France |
| |
|
| Platform ID |
GPL5175 |
| Series (2) |
| GSE37414 |
Expression of genetic adaptability of cancer cells under treatment selection pressure in multiple myeloma patients |
| GSE37469 |
Minor clone provides a reservoir for relapse in multiple myeloma |
|
