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Sample GSM98284

Query DataSets for GSM98284

Status

Public on Feb 23, 2007

Title

40%sugar_HS_2

Sample type

RNA

Source name

mRNA

Organism

Saccharomyces cerevisiae

Characteristics

Commercial baker's yeast specilized for use in high-sugar dough.

Extracted molecule

polyA RNA

Extraction protocol

Each samples of total RNA were extracted using the hot phenol method. Poly (A)+ RNA was isolated from the total RNA by using an Oligotex® dt30 (super) mRNA purification kit (Takara, Japan) according to the manufacturer’s instructions.

Label

Biotin

Label protocol

Labeling was done according to the Affymetrix GeneChip® Expression Analysis Technical Manual.

Description

After 60min fermentation using 40% sucrose LF medium.

Data processing

Affymetrix GeneChip Operating Software Version 1.2

Submission date

Feb 23, 2006

Last update date

Feb 27, 2006

Contact name

Jun Shima

E-mail(s)

shimaj@nfri.affrc.go.jp

Organization name

National Food Research Institute

Department

Applied Microbiology

Lab

Yeast

Street address

1-2-12 Kannondai

City

Tsukuba

State/province

Ibaraki

ZIP/Postal code

305-8642

Country

Japan

Platform ID

GPL2529

Series (1)

GSE4295

Sugar stress during fermentation (baker's yeast)

Data table header descriptions
ID_REF
VALUE	Normalized value (Per Chip, Per Gene)
BKD_RAW	Control signal (calculated :GeneSpring)
SIGNAL_RAW	Raw intensities (measured: Affymetrix GeneChip)
ABS_CALL	Flag (Affymetrix)

Data table
ID_REF	VALUE	BKD_RAW	SIGNAL_RAW	ABS_CALL
1769308_at	3.84	94.51	363	P
1769309_at	0.231	27.71	6.41	A
1769310_at	0.176	27.71	4.877	A
1769311_at	0.799	2,010	1,605	P
1769312_at	0.362	244.9	88.66	P
1769313_at	0.318	158.5	50.37	P
1769314_at	0.821	3,965	3,256	P
1769315_at	0.123	27.71	3.407	A
1769316_s_at	0.0198	27.71	0.549	A
1769317_at	0.869	961.6	835.3	P
1769318_at	0.188	27.71	5.218	A
1769319_at	0.598	767	458.4	P
1769320_at	0.56	356.1	199.6	P
1769321_at	1.616	512.3	827.6	P
1769322_s_at	0.0813	383.5	31.18	P
1769323_at	1.035	753	779.2	P
1769324_at	0.999	426.3	425.7	P
1769325_at	0.596	49.79	29.66	P
1769326_at	0.0446	27.71	1.237	A
1769327_at	0.129	27.71	3.565	A

Total number of rows: 10928

Table truncated, full table size 332 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM98284.CEL.gz	1.5 Mb	(ftp)(http)	CEL
GSM98284.CHP.gz	57.4 Kb	(ftp)(http)	CHP
Processed data provided as supplementary file