REV1 deoxycytidyl transferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: REV1
Gene description: deoxycytidyl transferase
Primary source: FungiDB:YOR346W
Locus tag: YOR346W
See related: SGD:S000005873; AllianceGenome:SGD:S000005873; VEuPathDB:YOR346W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA-directed DNA polymerase activity and deoxycytidyl transferase activity. Involved in error-free translesion synthesis and error-prone translesion synthesis. Located in mitochondrion and replication fork. Colocalizes with chromatin. Orthologous to human REV1 (REV1 DNA directed polymerase). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See REV1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (981828..984785)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Bypass of DNA interstrand crosslinks by a Rev1-DNA polymerase zeta complex.
Title: Bypass of DNA interstrand crosslinks by a Rev1-DNA polymerase ζ complex.
Structure-guided mutagenesis highlights the importance of UBM2 for REV1-mediated mutagenesis and DNA damage tolerance.
Title: Structural Basis for the Interaction of Mutasome Assembly Factor REV1 with Ubiquitin.
The structures of the translesion DNA synthesis polymerase Rev1 in complex with benzo[a]pyrene guanine adducts gives insights how Rev1 achieves error-free replication.
Title: Mechanism of error-free replication across benzo[a]pyrene stereoisomers by Rev1 DNA polymerase.
TLS polymerase REV1 carries out DNA lesion bypass only after the Def1-assisted removal of Pol3 from the stalled replication fork.
Title: Def1 promotes the degradation of Pol3 for polymerase exchange to occur during DNA-damage--induced mutagenesis in Saccharomyces cerevisiae.
Rev1-BRCT domain bound to PCNA can be displaced by increasing amounts of the PIP-box peptide.
Title: NMR mapping of PCNA interaction with translesion synthesis DNA polymerase Rev1 mediated by Rev1-BRCT domain.
Rev1 stably interacts with Rad5 via the C-terminal region of Rev1 and the N-terminal region of Rad5.
Title: A non-catalytic function of Rev1 in translesion DNA synthesis and mutagenesis is mediated by its stable interaction with Rad5.
The study reports the X-ray crystal structure of the yeast Rev1 BRCT domain and show that substitutions in residues constituting its phosphate-binding pocket do not affect mutagenic translesion synthesis.
Title: Structure and functional analysis of the BRCT domain of translesion synthesis DNA polymerase Rev1.
the levels of Rev1 protein are dependent on proteasome-mediated degradation;proteasomal degradation acts as a regulatory system of mutagenic translesion DNA synthesis mediated by Rev1.
Title: Proteasomal regulation of the mutagenic translesion DNA polymerase, Saccharomyces cerevisiae Rev1.
results show that Rev1's catalytic activity is important in vivo when the cell has to cope with specific DNA lesions, such as N(2)-dG.
Title: The DNA polymerase activity of Saccharomyces cerevisiae Rev1 is biologically significant.
We present here the structure of yeast Rev1 in ternary complex with DNA containing an abasic lesion and with dCTP as the incoming nucleotide. The structure shows a mechanism of synthesis across an abasic lesion that differs from that in other polymerases.
Title: DNA synthesis across an abasic lesion by yeast REV1 DNA polymerase.

Submit: New GeneRIF Correction See all GeneRIFs (27)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables damaged DNA binding	IEA Inferred from Electronic Annotation more info
enables deoxycytidyl transferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables deoxycytidyl transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotidyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in error-free translesion synthesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in error-free translesion synthesis	IDA Inferred from Direct Assay more info	PubMed
involved_in error-free translesion synthesis	IEA Inferred from Electronic Annotation more info
involved_in error-free translesion synthesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in error-prone translesion synthesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in error-prone translesion synthesis	IDA Inferred from Direct Assay more info	PubMed
involved_in error-prone translesion synthesis	IEA Inferred from Electronic Annotation more info
involved_in error-prone translesion synthesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in error-prone translesion synthesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in replication fork	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: deoxycytidyl transferase

NP_014991.1

Bifunctional DNA-directed DNA polymerase/deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress; may be involved in meiosis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

981828..984785

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183766.1 → NP_014991.1 TPA: deoxycytidyl transferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014991.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W341, P12689, Q12323

UniProtKB/TrEMBL

A6ZPH6, B3LK03, C7GNS2, N1NW89

Conserved Domains (3) summary

cd01701
Location:315 → 735

PolY_Rev1; DNA polymerase Rev1

cd12145
Location:875 → 981

Rev1_C; C-terminal domain of the Y-family polymerase Rev1

cd17719
Location:165 → 249

BRCT_Rev1; BRCT domain of DNA repair protein Rev1 and similar proteins