IRA2 Ras GTPase activating protein IRA2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: IRA2
Official Full Name: Ras GTPase activating protein IRA2
Primary source: SGD:S000005441
Locus tag: YOL081W
See related: AllianceGenome:SGD:S000005441; FungiDB:YOL081W; VEuPathDB:YOL081W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CCS1; GLC4
Summary: Enables GTPase activator activity. Involved in several processes, including cellular response to cold; cellular response to hydrogen peroxide; and positive regulation of GTPase activity. Located in endoplasmic reticulum membrane and mitochondrion. Used to study neurofibromatosis and neurofibromatosis 1. Human ortholog(s) of this gene implicated in autistic disorder; hematologic cancer (multiple); nervous system cancer (multiple); neurofibromatosis (multiple); and neurofibromatosis-Noonan syndrome. Orthologous to several human genes including NF1 (neurofibromin 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See IRA2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (171070..180309)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Crosstalk between protein kinase A and the HOG pathway under impaired biosynthesis of complex sphingolipids in budding yeast.
Title: Crosstalk between protein kinase A and the HOG pathway under impaired biosynthesis of complex sphingolipids in budding yeast.
IRA2 mutation reveals the phenotypic effects of the cryptic variants by disrupting the transcriptional silencing of one or more genes that contribute to the trait.We also show that this ability to disrupt FLO11 repression is not unique to IRA2
Title: Transcriptional Derepression Uncovers Cryptic Higher-Order Genetic Interactions.
the physiological relevance of the interaction of both Ira2 and Hsp60 with Bcy1. Bcy1 interacts with Ira2 tethering PKA to the Ras complex and Hsp60 chaperone localizes PKA to mitochondria and has a role in the kinase stability.
Title: Interacting proteins of protein kinase A regulatory subunit in Saccharomyces cerevisiae.
particular, the effect of the negative feedback loops on the activity of both Ira2 (a Ras-GAP) and Cdc25 (a Ras-GEF) proteins.
Title: Simulation of the Ras/cAMP/PKA pathway in budding yeast highlights the establishment of stable oscillatory states.
These findings provide evidence for conserved ubiquitination pathways regulating the RasGAP proteins Ira2 (in yeast) and neurofibromin (in humans).
Title: The RasGAP proteins Ira2 and neurofibromin are negatively regulated by Gpb1 in yeast and ETEA in humans.
Ira1 and Ira2 affect glycerophosphoinositol production and transport.
Title: Neurofibromin homologs Ira1 and Ira2 affect glycerophosphoinositol production and transport in Saccharomyces cerevisiae.
determinants of Tfs1p involved in the Tfs1/Ira2 interaction, the 3D structure of Ylr179cp have been modelled
Title: Molecular basis of the Tfs1/Ira2 interaction: a combined protein engineering and molecular modelling study.
Suppression of the snf3 mutant phenotype by deletion of IRA2 was confirmed.
Title: Loss of IRA2 suppresses the growth defect on low glucose caused by the snf3 mutation in Saccharomyces cerevisiae.
Gpb1/2 bind to a conserved C-terminal domain of Ira1/2, and loss of Gpb1/2 results in a destabilization of Ira1 and Ira2, leading to elevated levels of Ras2-GTP and unbridled cAMP-PKA signaling.
Title: The kelch proteins Gpb1 and Gpb2 inhibit Ras activity via association with the yeast RasGAP neurofibromin homologs Ira1 and Ira2.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables GTPase activator activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of Ras protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Ras GTPase activating protein IRA2

NP_014560.1

GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; IRA2 has a paralog, IRA1, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

171070..180309

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183335.1 → NP_014560.1 TPA: Ras GTPase activating protein IRA2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014560.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1Y7, O13592, P19158, Q08239

UniProtKB/TrEMBL

G2WMF4

Conserved Domains (1) summary

cd05392
Location:1672 → 1990

RasGAP_Neurofibromin_like; Ras-GTPase Activating Domain of proteins similar to neurofibromin