U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    CFD1 iron-sulfur cluster assembly protein CFD1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854814, updated on 29-Jun-2024

    Summary

    Go to the top of the page Help
    Gene symbol
    CFD1
    Gene description
    iron-sulfur cluster assembly protein CFD1
    Primary source
    FungiDB:YIL003W
    Locus tag
    YIL003W
    See related
    SGD:S000001265; AllianceGenome:SGD:S000001265; VEuPathDB:YIL003W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    DRE3
    Summary
    Enables 4 iron, 4 sulfur cluster binding activity. Contributes to ATP hydrolysis activity. Involved in iron-sulfur cluster assembly and tRNA wobble uridine modification. Located in cytoplasm. Part of Nbp35-Cfd1 ATPase complex. Orthologous to human NUBP2 (nucleotide binding protein 2). [provided by Alliance of Genome Resources, Apr 2022]
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See CFD1 in Genome Data Viewer
    Location:
    chromosome: IX
    Exon count:
    1
    Sequence:
    Chromosome: IX; NC_001141.2 (349122..350003)

    Chromosome IX - NC_001141.2Genomic Context describing neighboring genes Neighboring gene protein disulfide isomerase EPS1 Neighboring gene Bet1p Neighboring gene Cmi7p Neighboring gene phosphoinositide 5-phosphatase INP51

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_001141.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Coupling Nucleotide Binding and Hydrolysis to Iron-Sulfur Cluster Acquisition and Transfer Revealed through Genetic Dissection of the Nbp35 ATPase Site. Grossman JD, et al. Biochemistry, 2019 Apr 16. PMID 30865432
    2. The Cfd1 Subunit of the Nbp35-Cfd1 Iron Sulfur Cluster Scaffolding Complex Controls Nucleotide Binding. Grossman JD, et al. Biochemistry, 2019 Mar 26. PMID 30785732
    3. Approaches to Interrogate the Role of Nucleotide Hydrolysis by Metal Trafficking NTPases: The Nbp35-Cfd1 Iron-Sulfur Cluster Scaffold as a Case Study. Grossman JD, et al. Methods Enzymol, 2018. PMID 29746244
    4. The Yeast Nbp35-Cfd1 Cytosolic Iron-Sulfur Cluster Scaffold Is an ATPase. Camire EJ, et al. J Biol Chem, 2015 Sep 25. PMID 26195633, Free PMC Article
    5. Interaction with Cfd1 increases the kinetic lability of FeS on the Nbp35 scaffold. Pallesen LJ, et al. J Biol Chem, 2013 Aug 9. PMID 23798678, Free PMC Article

    See all (36) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. In the Nbp35-Cfd1 complex, Cfd1 protein binds ATP. Cfd1 protein is hydrolysis competent when bound to Nbp35.
      Title: The Cfd1 Subunit of the Nbp35-Cfd1 Iron Sulfur Cluster Scaffolding Complex Controls Nucleotide Binding.
    2. The Yeast Nbp35-Cfd1 Cytosolic Iron-Sulfur Cluster Scaffold Is an ATPase.
      Title: The Yeast Nbp35-Cfd1 Cytosolic Iron-Sulfur Cluster Scaffold Is an ATPase.
    3. a model in which Cfd1-Nbp35 interaction increases the lability of assembled FeS on the Nbp35 scaffold for transfer to target apo-FeS proteins.
      Title: Interaction with Cfd1 increases the kinetic lability of FeS on the Nbp35 scaffold.
    4. a model for the mode of Fe-S cluster coordination within the Cfd1-Nbp35 complex, explaining its specific properties as a Fe-S scaffold device.
      Title: A bridging [4Fe-4S] cluster and nucleotide binding are essential for function of the Cfd1-Nbp35 complex as a scaffold in iron-sulfur protein maturation.
    5. suggest that the Cfd1-Nbp35 complex functions as a novel scaffold for [Fe-S] cluster assembly in the eukaryotic cytosol
      Title: The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol.
    Submit: New GeneRIF Correction

    Pathways from PubChem

    Go to the top of the page

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP-dependent FeS chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron-sulfur cluster binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in iron-sulfur cluster assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in iron-sulfur cluster assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in iron-sulfur cluster assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in iron-sulfur cluster assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA wobble uridine modification IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of cytosolic [4Fe-4S] assembly scaffold complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    iron-sulfur cluster assembly protein CFD1
    NP_012263.1
    • Highly conserved iron-sulfur cluster binding protein; localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001141.2 Reference assembly

      Range
      349122..350003
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179353.1 → NP_012263.1  TPA: iron-sulfur cluster assembly protein CFD1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012263.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VVS7, P40558
      UniProtKB/TrEMBL
      A0A8H4F9F8, A6ZVR5, B3LTL2, B5VKT3, C7GJH5, C8ZAP6, N1P971
      Conserved Domains (1) summary
      pfam10609
      Location:15 → 271
      ParA; NUBPL iron-transfer P-loop NTPase
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P40558.1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
    Molecular Biology Databases
    Research Materials
    Tools