MSL5 mRNA splicing protein MSL5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MSL5
Official Full Name: mRNA splicing protein MSL5
Primary source: SGD:S000004106
Locus tag: YLR116W
See related: AllianceGenome:SGD:S000004106; FungiDB:YLR116W; VEuPathDB:YLR116W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables pre-mRNA branch point binding activity. Involved in mRNA splicing, via spliceosome. Located in cytosol and nucleus. Part of commitment complex. Orthologous to human SF1 (splicing factor 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See MSL5 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (380822..382252)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We also identify interfacial mutations in Msl5 that bypass the essentiality of Sub2, a DExD-box ATPase implicated in displacing Msl5 from the branchpoint in exchange for the U2 snRNP
Title: Structural basis for recognition of intron branchpoint RNA by yeast Msl5 and selective effects of interfacial mutations on splicing of yeast pre-mRNAs.
The results suggest a network of important but functionally buffered protein-protein and protein-RNA interactions between the Mud2-Msl5 complex at the branchpoint and the U1 snRNP at the 5' splice site.
Title: Structure-function analysis and genetic interactions of the yeast branchpoint binding protein Msl5.
a conserved zinc knuckle of the branch point-binding protein plays a different role in RNA processing than enhancing the binding of branch point-binding protein to the branch point sequence
Title: An extended RNA binding site for the yeast branch point-binding protein and the role of its zinc knuckle domains in RNA binding.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables pre-mRNA branch point binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA splicing, via spliceosome	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of mRNA splicing, via spliceosome	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of commitment complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mRNA splicing protein MSL5

NP_013217.1

Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

380822..382252

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182003.1 → NP_013217.1 TPA: mRNA splicing protein MSL5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013217.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYB4, Q12186

UniProtKB/TrEMBL

C7GQD5, G2WIV8

Conserved Domains (3) summary

PHA03247
Location:363 → 435

PHA03247; large tegument protein UL36; Provisional

COG5082
Location:267 → 386

AIR1; Arginine methyltransferase-interacting protein, contains RING Zn-finger [Posttranslational modification, protein turnover, chaperones / Intracellular trafficking and secretion]

COG5176
Location:1 → 269

MSL5; Splicing factor (branch point binding protein) [RNA processing and modification]