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    INO2 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851701, updated on 11-Sep-2024

    Summary

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    Official Symbol
    INO2
    Primary source
    SGD:S000002530
    Locus tag
    YDR123C
    See related
    AllianceGenome:SGD:S000002530; FungiDB:YDR123C; VEuPathDB:YDR123C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    DIE1; SCS1
    Summary
    Contributes to DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in phospholipid biosynthetic process and positive regulation of transcription by RNA polymerase II. Located in nucleus. Part of RNA polymerase II transcription regulator complex. [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (698554..699468, complement)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene DNA polymerase epsilon noncatalytic subunit Neighboring gene serine/threonine protein kinase KIN1 Neighboring gene uncharacterized protein Neighboring gene alpha/beta hydrolase family protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Tailoring the Saccharomyces cerevisiae endoplasmic reticulum for functional assembly of terpene synthesis pathway. Kim JE, et al. Metab Eng, 2019 Dec. PMID 31445083
    2. IN02, a positive regulator of lipid biosynthesis, is essential for the formation of inducible membranes in yeast. Block-Alper L, et al. Mol Biol Cell, 2002 Jan. PMID 11809821, Free PMC Article
    3. Over-expression of the INO2 regulatory gene alters regulation of an INO1-lacZ reporter gene but does not affect regulation of INO1 expression. Miller LL, et al. Curr Genet, 2001 Apr. PMID 11405099
    4. Expression of the INO2 regulatory gene of Saccharomyces cerevisiae is controlled by positive and negative promoter elements and an upstream open reading frame. Eiznhamer DA, et al. Mol Microbiol, 2001 Mar. PMID 11251853
    5. Functional characterization of the INO2 gene of Saccharomyces cerevisiae. A positive regulator of phospholipid biosynthesis. Nikoloff DM, et al. J Biol Chem, 1994 Mar 11. PMID 8125958

    See all (121) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural Analysis of Ino2p/Ino4p Mutual Interactions and Their Binding Interface with Promoter DNA.
      Title: Structural Analysis of Ino2p/Ino4p Mutual Interactions and Their Binding Interface with Promoter DNA.
    2. corepressors may be recruited not only by repressors but also by activators such as Ino2. Indeed, we could demonstrate direct interaction of Ino2 with Sin3 and Cyc8. The Opi1 repressor interaction domain within Ino2 is also able to contact Sin3 and Cyc8.
      Title: Promoter recruitment of corepressors Sin3 and Cyc8 by activator proteins of the yeast Saccharomyces cerevisiae.
    3. Upon induction, Ino2p dissociates from the INM1 promoter.
      Title: The transcriptional activator Ino2p dissociates from the yeast INM1 promoter in induction.
    4. Cbf1p binding to the upstream sites was required for the binding of the ISW2 chromatin-remodeling complex to the Ino2p-Ino4p-binding sites on the INO1 promoter.
      Title: Derepression of INO1 transcription requires cooperation between the Ino2p-Ino4p heterodimer and Cbf1p and recruitment of the ISW2 chromatin-remodeling complex.
    5. Factors required for activation of yeast phospholipid biosynthetic genes depend on activator protein Ino2 which binds to the inositol/choline-responsive element.
      Title: Mediator subunits and histone methyltransferase Set2 contribute to Ino2-dependent transcriptional activation of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae.
    6. Ino2p activator is required for histone acetylation.
      Title: Gene-wide histone acetylation at the yeast INO1 requires the transcriptional activator Ino2p.
    7. Autoregulatory expression of INO2, INO4 and OPI1 was abolished by promoter exchange experiments, showing that autoregulation of regulators contributes to the degree of differential gene expression but is not responsible for it.
      Title: Dimerization of yeast transcription factors Ino2 and Ino4 is regulated by precursors of phospholipid biosynthesis mediated by Opi1 repressor.
    8. Genes containing the UASINO sequence were bound by Ino2p.
      Title: Genome-wide analysis reveals inositol, not choline, as the major effector of Ino2p-Ino4p and unfolded protein response target gene expression in yeast.
    9. Gene repression occurs in the presence of sufficient inositol and choline, requiring an intact Opi1 repressor which binds to Ino2, we mapped an 18 aa repressor interaction domain within Ino2 necessary and sufficient for binding by Opi1.
      Title: Constitutive expression of yeast phospholipid biosynthetic genes by variants of Ino2 activator defective for interaction with Opi1 repressor.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to DNA-binding transcription activator activity, RNA polymerase II-specific IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    contributes_to DNA-binding transcription activator activity, RNA polymerase II-specific IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    contributes_to RNA polymerase II cis-regulatory region sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein dimerization activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in phospholipid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in phospholipid biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of RNA polymerase II transcription regulator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of RNA polymerase II transcription regulator complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Ino2p
    NP_010408.1
    • Transcription factor; component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion; involved in diauxic shift

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      698554..699468 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180431.1 → NP_010408.1  TPA: Ino2p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010408.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VSA9, P26798
      UniProtKB/TrEMBL
      A6ZY85, B3LGF3, B5VG18, C7GIN1, C8Z539, G2WAM5, N1P6C7
      Conserved Domains (1) summary
      cd11388
      Location:235 → 302
      bHLH_ScINO2_like; basic helix-loop-helix (bHLH) domain found in Saccharomyces cerevisiae protein INO2 and similar proteins
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P26798.1

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