CLB2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CLB2
Primary source: SGD:S000006323
Locus tag: YPR119W
See related: AllianceGenome:SGD:S000006323; FungiDB:YPR119W; VEuPathDB:YPR119W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables cyclin-dependent protein serine/threonine kinase regulator activity. Involved in several processes, including G2/M transition of mitotic cell cycle; negative regulation of protein dephosphorylation; and positive regulation of cell cycle. Located in several cellular components, including cellular bud neck; microtubule cytoskeleton; and nucleus. Human ortholog(s) of this gene implicated in breast cancer and prostate cancer. Orthologous to several human genes including CCNB1 (cyclin B1) and CCNB2 (cyclin B2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (771653..773128)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Forkhead transcription factor Fkh1: insights into functional regulatory domains crucial for recruitment of Sin3 histone deacetylase complex.
Title: Forkhead transcription factor Fkh1: insights into functional regulatory domains crucial for recruitment of Sin3 histone deacetylase complex.
Data indicate the role of the principal mitotic cyclin gene CLB2 at the end of S phase.
Title: Genetic Evidence for Roles of Yeast Mitotic Cyclins at Single-Stranded Gaps Created by DNA Replication.
Our observations suggest that both APC(Cdh1) and SCF(Cdc4)-dependent proteolysis of Clb6 at the G1/S border are crucial for multiple cell cycle regulated events including proper expression of Clb2, the G1/S and G2/M cell cycle transitions and for proper completion of cell division at mitotic exit.
Title: The anaphase-promoting complex works together with the SCF complex for proteolysis of the S-phase cyclin Clb6 during the transition from G1 to S phase.
Cdk1 and multiple cyclins become highly enriched at DSBs and that the recruitment of Cdk1 and cyclins Clb2 and Clb5 ensures optimal Fun30 phosphorylation and checkpoint activation.
Title: Enrichment of Cdk1-cyclins at DNA double-strand breaks stimulates Fun30 phosphorylation and DNA end resection.
Size control seems to be exerted twice, where the G2/M control affects bud size through bud-localized translation of CLB2 mRNA, explaining the dependence of the S-G2-M duration on nutrients.
Title: Bud-Localization of CLB2 mRNA Can Constitute a Growth Rate Dependent Daughter Sizer.
Models for the functional interactions of mitotic cyclin Clb2, Sgs1 and Exo1 with replication fork stabilization are proposed.
Title: The analysis of S. cerevisiae cells deleted for mitotic cyclin Clb2 reveals a novel requirement of Sgs1 DNA helicase and Exonuclease 1 when replication forks break in the presence of alkylation damage.
The essential role of nuclear Clb2 is in both survival and adaptation to the DNA damage checkpoint, illustrating a spatially distinct dual function of this mitotic cyclin in the response to DNA damage.
Title: Spatially distinct functions of Clb2 in the DNA damage response.
Study discovered a pathway that links starvation to control of CLB2 mRNA stability and M phase delay. Dbf2 phosphorylates and activates the arginine methyltransferase Hmt1. In turn, Hmt1 methylates hnRNPs, promoting their nuclear localization, their cotranscriptional association with CLB2 mRNA, and the stability of CLB2 mRNA.
Title: A nutrient-responsive pathway that determines M phase timing through control of B-cyclin mRNA stability.
inactivation of the negative regulatory system by deletion of the HIR1 gene relieved the dependence of HTA1-HTB1 transcription on the mitotic Clb1/Cb2 cyclins
Title: The mitotic Clb cyclins are required to alleviate HIR-mediated repression of the yeast histone genes at the G1/S transition.
We found that the transcriptional cofactor Swi6p and activator Swi4p, as well as the G(2)/M-specific cyclin Clb2p, are required in a microtubule-destabilizing environment
Title: Cell cycle regulators interact with pathways that modulate microtubule stability in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables cyclin-dependent protein serine/threonine kinase regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables cyclin-dependent protein serine/threonine kinase regulator activity	IDA Inferred from Direct Assay more info	PubMed
enables cyclin-dependent protein serine/threonine kinase regulator activity	IEA Inferred from Electronic Annotation more info
enables cyclin-dependent protein serine/threonine kinase regulator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G2/M transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in mitotic cell cycle phase transition	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cell cycle phase transition	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein dephosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of mitotic spindle pole body separation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cyclin-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
part_of cyclin-dependent protein kinase holoenzyme complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle pole body	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole body	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: B-type cyclin CLB2

NP_015444.1

B-type cyclin involved in cell cycle progression; also involved in response to DNA damage; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB2 has a paralog, CLB1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

771653..773128

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184216.1 → NP_015444.1 TPA: B-type cyclin CLB2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015444.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W4B8, P24869

UniProtKB/TrEMBL

A6ZX00, B3LK83, B5VTQ9, C7GLI2, C8ZJE1, G2WPT6, N1NWG9

Conserved Domains (1) summary

COG5024
Location:8 → 487

COG5024; Cyclin [Cell division and chromosome partitioning]