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    TPT1 tRNA 2'-phosphotransferase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854051, updated on 29-Jun-2024

    Summary

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    Gene symbol
    TPT1
    Gene description
    tRNA 2'-phosphotransferase
    Primary source
    FungiDB:YOL102C
    Locus tag
    YOL102C
    See related
    SGD:S000005462; AllianceGenome:SGD:S000005462; VEuPathDB:YOL102C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables tRNA 2'-phosphotransferase activity. Involved in tRNA splicing, via endonucleolytic cleavage and ligation. Located in cytoplasm and nucleus. Orthologous to human TRPT1 (tRNA phosphotransferase 1). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See TPT1 in Genome Data Viewer
    Location:
    chromosome: XV
    Exon count:
    1
    Sequence:
    Chromosome: XV; NC_001147.6 (125997..126689, complement)

    Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene gag protein Neighboring gene gag-pol fusion protein Neighboring gene myo-inositol transporter ITR2 Neighboring gene Izh4p Neighboring gene serine/threonine protein kinase PKH2

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001147.6

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1. Sawaya R, et al. RNA, 2005 Jan. PMID 15611301, Free PMC Article
    2. Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism. Steiger MA, et al. RNA, 2005 Jan. PMID 15611300, Free PMC Article
    3. A conditional lethal yeast phosphotransferase (tpt1) mutant accumulates tRNAs with a 2'-phosphate and an undermodified base at the splice junction. Spinelli SL, et al. RNA, 1997 Dec. PMID 9404890, Free PMC Article
    4. A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae. Culver GM, et al. J Biol Chem, 1997 May 16. PMID 9148937
    5. An NAD derivative produced during transfer RNA splicing: ADP-ribose 1"-2" cyclic phosphate. Culver GM, et al. Science, 1993 Jul 9. PMID 8392224

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural and biochemical insights into the molecular mechanism of TRPT1 for nucleic acid ADP-ribosylation.
      Title: Structural and biochemical insights into the molecular mechanism of TRPT1 for nucleic acid ADP-ribosylation.
    2. identified four residues-Arg23, His24, Arg71, and Arg138-as essential for Tpt1 function in vivo
      Title: Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1.
    3. examined 21 Tpt1 protein variants mutated in conserved residues or blocks of residues and show that one of them, Tpt1 K69A/R71S protein, accumulates large amounts of intermediate with trinucleotide substrate due to a very slow second step
      Title: Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables tRNA 2'-phosphotransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables tRNA 2'-phosphotransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA 2'-phosphotransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA splicing, via endonucleolytic cleavage and ligation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tRNA splicing, via endonucleolytic cleavage and ligation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    tRNA 2'-phosphotransferase
    NP_014539.1
    • tRNA 2'-phosphotransferase; catalyzes the final step in tRNA splicing, transferring the 2'-PO(4) from the splice junction to NAD(+), forming ADP-ribose 1''-2''cyclic phosphate and nicotinamide; ADP-ribosyltransferase that ADP-ribosylates 5'-phosphorylated ssDNA and ssRNAs; human and mouse orthologs complement the inviability of the yeast null mutant

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001147.6 Reference assembly

      Range
      125997..126689 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001183356.1 → NP_014539.1  TPA: tRNA 2'-phosphotransferase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014539.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W1W6, Q12272
      UniProtKB/TrEMBL
      A0A8H4BWQ4, G2WMD3, N1NX51
      Conserved Domains (1) summary
      pfam01885
      Location:9 → 190
      PTS_2-RNA; RNA 2'-phosphotransferase, Tpt1 / KptA family
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q12272.1

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