WHI5 transcriptional repressor WHI5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: WHI5
Gene description: transcriptional repressor WHI5
Primary source: FungiDB:YOR083W
Locus tag: YOR083W
See related: SGD:S000005609; AllianceGenome:SGD:S000005609; VEuPathDB:YOR083W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables RNA polymerase II-specific DNA-binding transcription factor binding activity and transcription corepressor activity. Involved in negative regulation of transcription involved in G1/S transition of mitotic cell cycle; response to extracellular stimulus; and traversing start control point of mitotic cell cycle. Located in cytoplasm and nucleus. Part of SBF transcription complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See WHI5 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (479533..480420)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The G1/S repressor WHI5 is expressed at similar levels throughout the cell cycle.
Title: The G1/S repressor WHI5 is expressed at similar levels throughout the cell cycle.
Growth-dependent signals drive an increase in early G1 cyclin concentration to link cell cycle entry with cell growth.
Title: Growth-dependent signals drive an increase in early G1 cyclin concentration to link cell cycle entry with cell growth.
Cell Cycle Inhibitor Whi5 Records Environmental Information to Coordinate Growth and Division in Yeast.
Title: Cell Cycle Inhibitor Whi5 Records Environmental Information to Coordinate Growth and Division in Yeast.
Cell-size regulation in budding yeast does not depend on linear accumulation of Whi5.
Title: Cell-size regulation in budding yeast does not depend on linear accumulation of Whi5.
Whi5 phosphorylation by Cln3-Cdk1 embedded in the G1/S network dynamically controls critical cell size and cell fate.
Title: Whi5 phosphorylation embedded in the G1/S network dynamically controls critical cell size and cell fate.
at its most fundamental level, size control in budding yeast results from the differential scaling of Cln3 and Whi5 synthesis rates with cell size
Title: Dilution of the cell cycle inhibitor Whi5 controls budding-yeast cell size.
Hog1 interacts with and phosphorylates components of the core cell cycle transcriptional machinery such as Whi5 and the coregulator Msa1. Phosphorylation of these two transcriptional regulators by Hog1 is essential for inhibition of G1 cyclin expression.
Title: Hog1 targets Whi5 and Msa1 transcription factors to downregulate cyclin expression upon stress.
study describes a protein motif, the GTB motif (for G1/S transcription factor binding), in Nrm1 and Whi5 that is required to bind to the transcription factors
Title: Repression of G1/S transcription is mediated via interaction of the GTB motifs of Nrm1 and Whi5 with Swi6.
Cln3 regulates SBF by at least two different pathways, one involving the repressive protein Whi5, and the second involving Stb1.
Title: Recruitment of Cln3 cyclin to promoters controls cell cycle entry via histone deacetylase and other targets.
In a manner analogous to cyclin D/CDK4/6, which phosphorylates Rb in mammalian cells disrupting its association with HDACs, phosphorylation by the early G1 CDKs Cln3-Cdc28 and Pcl9-Pho85 inhibits association of Whi5 with the HDACs.
Title: Dual regulation by pairs of cyclin-dependent protein kinases and histone deacetylases controls G1 transcription in budding yeast.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA polymerase II-specific DNA-binding transcription factor binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription coregulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription corepressor activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription corepressor activity	IGI Inferred from Genetic Interaction more info	PubMed
enables transcription corepressor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in G1/S transition of mitotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in G1/S transition of mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in G1/S transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell size	HMP more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in traversing start control point of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of SBF transcription complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SBF transcription complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: transcriptional repressor WHI5

NP_014726.1

Repressor of G1 transcription; binds to SCB binding factor (SBF) at SCB target promoters in early G1; WHI5 expression is repressed in M/early G phase; phosphorylation of Whi5p by G1 cyclin (Cln)/Cdc28p relieves repression and promoter binding by Whi5p, drives nuclear export, and contributes to both the determination of critical cell size at START and cell fate; dephosphorylated and re-imported into the nucleus post-START during the starvation response, reversing commitment