TIF11 Tif11p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: TIF11
Gene description: Tif11p
Primary source: FungiDB:YMR260C
Locus tag: YMR260C
See related: SGD:S000004873; AllianceGenome:SGD:S000004873; VEuPathDB:YMR260C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables RNA binding activity; ribosomal small subunit binding activity; and translation initiation factor binding activity. Involved in translational initiation. Located in cytoplasmic stress granule. Part of eukaryotic 43S preinitiation complex and eukaryotic 48S preinitiation complex. Used to study uveal melanoma. Orthologous to human EIF1AX (eukaryotic translation initiation factor 1A X-linked) and EIF1AY (eukaryotic translation initiation factor 1A Y-linked). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See TIF11 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (789378..789839, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

eIF5-CTD (amino acids 241-405; Tif5p-B6) interacted with eIF1A (Tif11p).
Title: The interaction between eukaryotic initiation factor 1A and eIF5 retains eIF1 within scanning preinitiation complexes.
eIF1 autoregulates translation in yeast and discrimination against poor sequence context in vivo depends on specific domains and residues in eIF1, eIF1A, and eIF2beta
Title: Functional elements in initiation factors 1, 1A, and 2β discriminate against poor AUG context and non-AUG start codons.
A pair of repeats in the eIF1A CTT, dubbed Scanning Enhancer 1 (SE1) and SE2, was found to stimulate recruitment of Met-tRNA(i)(Met) in the ternary complex (TC) with eIF2.GTP and also to block initiation at UUG codons
Title: Regulatory elements in eIF1A control the fidelity of start codon selection by modulating tRNA(i)(Met) binding to the ribosome.
Following 80S complex formation, GTP hydrolysis by eIF5B enables the release of both eIF5B and eIF1A, and the ribosome enters the elongation phase of protein synthesis
Title: Coupled release of eukaryotic translation initiation factors 5B and 1A from 80S ribosomes following subunit joining.
The OB-fold is crucial for ribosome-binding and the C-terminal domain of eIF1A has eukaryotic-specific functions in ternary complex recruitment and scanning.
Title: The eIF1A C-terminal domain promotes initiation complex assembly, scanning and AUG selection in vivo.
Location of the mutations suggests that their mechanism of suppression may depend on effects on the conserved G-protein elements: the P-loop and nucleotide-binding element.
Title: Unique classes of mutations in the Saccharomyces cerevisiae G-protein translation elongation factor 1A suppress the requirement for guanine nucleotide exchange.
Data suggest strongly that the interaction between eIF1A and eIF5 is involved in maintaining the fidelity of start codon AUG recognition in vivo.
Title: Communication between eukaryotic translation initiation factors 5 and 1A within the ribosomal pre-initiation complex plays a role in start site selection.
N- and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection.
Title: N- and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection.
Our results suggest that eIF1 and eIF1A promote an open, scanning-competent preinitiation complex that closes upon start codon recognition and eIF1 release to stabilize ternary complex binding and clamp down on mRNA.
Title: The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables ribosomal small subunit binding	IDA Inferred from Direct Assay more info	PubMed
enables ribosomal small subunit binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables translation initiation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables translation initiation factor activity	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor activity	IEA Inferred from Electronic Annotation more info
enables translation initiation factor binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in formation of cytoplasmic translation initiation complex	IGI Inferred from Genetic Interaction more info	PubMed
involved_in formation of cytoplasmic translation initiation complex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in formation of translation initiation ternary complex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in formation of translation preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
involved_in formation of translation preinitiation complex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translation reinitiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translational initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translational initiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic stress granule	HDA more info	PubMed
part_of eukaryotic 43S preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
part_of eukaryotic 48S preinitiation complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Tif11p

NP_013987.1

Translation initiation factor eIF1A; essential protein that forms a complex with Sui1p (eIF1) and 40S ribosomal subunit and scans for start codons; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3; ortholog of bacterial translation initiation factor IF1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

789378..789839 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182767.1 → NP_013987.1 TPA: Tif11p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013987.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W086, P38912

UniProtKB/TrEMBL

A6ZMX5, B3LME7, C7GR92, C8ZFC6, G2WKW8, N1P5Y1

Conserved Domains (1) summary

cl09927
Location:17 → 132

S1_like; Ribosomal protein S1-like RNA-binding domain. Found in a wide variety of RNA-associated proteins. Originally identified in S1 ribosomal protein. This superfamily also contains the Cold Shock Domain (CSD), which is a homolog of the S1 domain. Both domains ...