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Fluorescence Binding Assays from Article 10.1021/acschembio.6b00720: "Molecular Mechanism for Isoform-Selective Inhibition of Acyl Protein Thioesterases 1 and 2 (APT1 and APT2)."
Assay data:3 Active, 2 Activity ≤ 1 µM, 4 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Steady-State Kinetic Assays from Article 10.1021/acschembio.6b00720: "Molecular Mechanism for Isoform-Selective Inhibition of Acyl Protein Thioesterases 1 and 2 (APT1 and APT2)."
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Enzymatic Protein Activity Assay from Article 10.1002/cbic.201200571: "Boron-based inhibitors of acyl protein thioesterases 1 and 2."
Assay data:14 Active, 5 Activity ≤ 1 µM, 18 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of FP-Rh binding to APT-1/2 in human A-431 cells at 1 mM preincubated for 2 hrs followed by compound washout and subsequent FP-Rh addition and measured after 1 hr by SDS-PAGE analysis
Assay data:2 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Binding affinity to HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) assessed as stoichiometric complex formation at 10 uM after 5 mins by electrospray ionization native mass spectrometry
Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay
Assay data:1 Active, 2 Tested
Binding affinity to APT2 in HEK293T cells at 50 uM incubated for 1 hr presence of generic lipase inhibitor HDFP by mass spectrometry analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to APT2 in HEK293T cells at 50 uM incubated for 1 hr by mass spectrometry analysis relative to azide-PEG3-biotin
Competitive inhibition of HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) preincubated for 30 mins followed by substrate addition by resorufin dye based acetate hydrolysis assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 4 Tested
Binding affinity to APT2 in HEK293T cells at 50 uM incubated for 1 hr by mass spectrometry analysis relative to o-ML349-biotin
Binding affinity to APT2 in HEK293T cells at 50 uM preincubated for 1 hr followed by FP-TAMRA labeling by in-gel fluorescence assay
Assay data:1 Active, 3 Tested
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
A screen to identify genes that regulate endothelial cell migration - TertiaryScreen
Assay data:111 Active, 153 Tested
A screen to identify genes that regulate endothelial cell migration - Morphology analysis
Assay data:153 Tested
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
SummaryPubMed Citation
A screen to identify genes that regulate lymphatic endothelial cell migration - Secondary Screen
Assay data:340 Active, 500 Tested
SummaryRelated BioAssays by Depositor
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
Identification of proteins required for the processing of the EWS-FLI1 nascent transcript
Assay data:59220 Tested
InfectX: Pathogen infection siRNA screens
Assay data:115372 Tested
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