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Binding affinity to human notum assessed as dissociation constant by thermal shift assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human notum transfected with HEK293 cell incubated for 16 hrs by luciferase assay
Inhibition of human notum using OTPS as substrate incubated for 16 hrs by fluorescent based assay
Inhibition of notum (unknown origin)
Biochemical Notum Enzymatic Activity Assay from US Patent US11634391: "Compounds which are inhibitors of Notum"
Assay data:192 Active, 133 Activity ≤ 1 µM, 253 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of human Notum (S81 to T451 residues) Cys330Ser mutant expressed in HEK293S cells at 100 uM using OPTS as substrate incubated for 40 mins by fluorescence based assay relative to control
Inhibition of human Notum (S81 to T451 residues) Cys330Ser mutant expressed in HEK293S cells at 10 uM using OPTS as substrate incubated for 40 mins by fluorescence based assay relative to control
Inhibition of notum in human SW620 cells
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of full length recombinant human Notum expressed in HEK293S cells expressing TCF-LEF preincubated for 30 mins followed by Wnt-3A addition and measured after 1 hr by luminescence based microplate reader analysis
Inhibition of human Notum (S81 to T451 residues) Cys330Ser mutant expressed in HEK293S cells using OPTS as substrate incubated for 40 mins by fluorescence based assay
Assay data:29 Active, 14 Activity ≤ 1 µM, 42 Tested
Binding affinity to human Notum (S81 to T451 residues) Cys330Ser mutant expressed in human HEK293T cells by SPR analysis
Assay data:2 Tested
Inhibition of human Notum (S81 to T451 residues) Cys330Ser mutant in human HEK293 cells expressing Renilla and STF reporter gene and measured after 24 hrs in presence of mouse L-Wnt3a cells by Dual-Glo luciferase reporter assay
Inhibition of human Notum (81 to T451 residues) Cys330Ser mutant using OPTS substrate incubated for 16 hrs by fluorescence based assay
Inhibition of human Notum using OPTS substrate incubated for 1 hr by fluorescence based assay
Assay data:6 Active, 6 Activity ≤ 1 µM, 7 Tested
Inhibition of human Notum using OPTS substrate by fluorescence based assay
Assay data:4 Active, 2 Activity ≤ 1 nM, 4 Activity ≤ 1 µM, 7 Tested
Inhibition of C-terminal His-V5 tagged human Notum expressed in human HEK293F cells harbouring TCF/LEF luciferase reporter gene assessed as activation of Wnt signaling using WNT3A as substrate by Steady-Glo luciferase assay
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Assay data:2 Active, 2 Activity ≤ 1 µM, 4 Tested
Inhibition of C-terminal His-tagged Notum (Ser81-Thr451 Cys330Ser) (unknown origin) assessed as restoration of Wnt/beta catenin signalling activated by exogenous recombinant Wnt by TCF/LCF reporter assay
Inhibition of C-terminal His-tagged Notum (Ser81-Thr451 Cys330Ser) (unknown origin) using OPTS as substrate by fluorescence based assay
Inhibition of Notum (81 to 451 Cys330Ser) in HEK293T cells expressing TCF-LEF assessed as activation of Wnt signaling using WNT3A as substrate incubated for 16 to 20 mins by Steady-Glo luciferase assay
Assay data:23 Active, 23 Activity ≤ 1 µM, 35 Tested
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