Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Z'-LYTE: ERBB2 (HER2), Direct Format, kinase activity assay, coupled enzyme activity measurement method, detected via fluorescence resonance energy transfer
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µM
Inhibition of HER2 (unknown origin)
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Induction of HER2 destabilization in human MCF7 cells assessed as half life at 10 uM measured up to 24 hrs by immunoblotting assay (Rvb > 24 hrs)
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human recombinant ERBB2 (537 to 636 residues) expressed in Escherichia coli using Tyr 06 as substrate after 1 hr in presence of ATP by Z'-LYTE assay
Inhibition of recombinant human His-tagged cytoplasmic ERBB2 expressed in baculovirus expression system by Z'-LYTE assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 4 Tested
Inhibition of human HER2 in human A549 cells by ELISA method
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Inhibition of recombinant ERBB2 (unknown origin) at 1 uM using poly (Glu,Tyr)4:1 as substrate incubated for 60 mins by ELISA
Assay data:2 Tested
Inhibition of HER2 (unknown origin) using FAM-labeled peptide substrate preincubated for 10 mins followed by substrate addition measured after 40 mins by caliper motility shift assay
Assay data:9 Active, 9 Activity ≤ 1 µM, 9 Tested
Inhibition of HER2 (unknown origin) at 1 uM
Inhibition of ERBB2 (unknown origin)
Inhibition of ERBB2 (unknown origin) at 1 uM relative to control
Inhibition of human ERBB2 expressed in mammalian system assessed as remaining enzyme activity at 1000 nM relative to untreated control
Binding affinity to wild-type human partial length ERBB2 (K646 to V1225 residues) expressed in mammalian expression system assessed as residual binding level at 10000 nM by Kinomescan method relative to control
Inhibition of HER2 (unknown origin) preincubated for 1 hr followed by Biotin-AVLESEEELYSSARQ-NH2 substrate addition in presence of ATP and measured after 1 hr by TR-FRET assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of human His-tagged ERBB2 expressed in baculovirus expression system assessed as reduction in enzyme activity at 5 uM in presence of ATP relative to control
Binding affinity to wild-type partial length human ERRB2 (K646 to V1225 residues) expressed in mammalian expression system by Kinomescan assay
Inhibition of ErbB2 (unknown origin) at 10 nM relative to control
Inhibition of ErbB2 (unknown origin) at 100 nM relative to control
Inhibition of ErbB2 (unknown origin) at 1000 nM relative to control
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on