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Binding affinity to 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli BL21 (DE3) in presence of acetylated p53pT387 peptide by crystallography
Assay data:3 Active, 3 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli at 1 mM by SPR assay
Assay data:1 Active, 1 Tested
Binding affinity to 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli at 1 mM by fluorescence polarization assay
Binding affinity to 15N2H labeled and 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli assessed as induction of broadening (intensity decrease) and chemical shift value perturbation at 2000 uM in presence of p53pT387 peptide by 1H-15N TROSY-HSQC NMR spectroscopy
Binding affinity to 15N2H labeled and 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli assessed as induction of broadening (intensity decrease) and chemical shift value perturbation at 2000 uM in absence of of p53pT387 peptide by 1H-15N TROSY-HSQC NMR spectroscopy
Binding affinity to 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli BL21 (DE3) at 500 uM in presence of p53pT387 peptide by WaterLOGSY NMR spectroscopy
Genes that modify the natural suppressive ability of myoepithelial cells: 3D co-culture siRNA screen
Assay data:18 Active, 83 Tested
Summary
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Binding affinity to SFN in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay
Inhibition of TMR-GG-RLSHpSSLPG-NH2 binding to recombinant human GST-tagged 14-3-3-sigma preincubated for 30 mins followed by TMR-GG-RLSHpSSLPG-NH2 addition and measured after 60 mins by fluorescence polarization assay
Assay data:5 Active, 5 Tested
Inhibition of 14-3-3sigma (unknown origin) assessed as reduction in 14-3-3sigma- mediated increase in MMP1 mRNA by cell based assay
Induction of human 14-3-3sigma binding to TASK-3 using FAM-370RRK-pS-V374-COOH by fluorescence polarization assay relative to control
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
SummaryPubMed Citation
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
SummaryRelated BioAssays by Depositor
Identification of proteins required for the processing of the EWS-FLI1 nascent transcript
Assay data:59220 Tested
InfectX: Pathogen infection siRNA screens
Assay data:115372 Tested
A whole genome RNAi screen highlights components of the ER/Golgi as a prominent source of resistance to immunotoxin-mediated cytotoxicity
Assay data:555 Active, 64752 Tested
RNAi screen for vorinostat resistance genes - Primary Screen
Assay data:450 Active, 18118 Tested
Druggable Genome siRNA Screen for Enhancers of Camptothecin in MDA-MB-231 Breast Cancer Cells
Assay data:167 Active, 26449 Tested
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