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HTS assay from US Patent US9247759: "Identification of human T2R receptors that respond to bitter compounds that elicit the bitter taste in compositions, and the use thereof in assays to identify compounds that inhibit (block) bitter taste in compositions and use thereof"
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by DepositorRelated BioAssays by Target
Fluorescence Polarization Assays from US Patent US9247759: "Identification of human T2R receptors that respond to bitter compounds that elicit the bitter taste in compositions, and the use thereof in assays to identify compounds that inhibit (block) bitter taste in compositions and use thereof"
Assay data:409 Active, 252 Activity ≤ 1 µM, 422 Tested
Activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R8 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as effect on chloramphenicol-induced intracellular calcium level at 100 uM by FLIPR assay
Assay data:2 Tested
SummaryRelated BioAssays by Target
Agonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R8 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as effect on intracellular calcium level at 100 uM by FLIPR assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Agonist activity at human TAS2R8 expressed in HEK293T cells co-expressing Galpha15 assessed as increase in intracellular calcium level at 20 mM by Calcium-3 dye based fluorescence assay relative to control
Assay data:3 Tested
Agonist activity at human TAS2R8 expressed in HEK293T cells co-expressing Galpha15 assessed as increase in intracellular calcium level by Calcium-3 dye based fluorescence assay
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Antagonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R8 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as inhibition of chloramphenicol-induced increase in intracellular calcium level at 100 uM by FLIPR assay
Agonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R8 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as increase in intracellular calcium level at 100 uM by FLIPR assay
Antagonist activity at recombinant human TAS2R8 transiently expressed in HEK293T co-expressing Galpha16gust44 assessed as inhibition of andrographolide-induced intracellular calcium level by measuring remaining activity at 25 uM by fluo-4AM dye based FLIPR assay relative to control
SummaryPubMed CitationRelated BioAssays by Target
Competitive antagonist activity at recombinant human TAS2R8 assessed as inhibition of andrographolide-induced intracellular calcium level at 3 to 3000 nM preincubated for 60 secs followed by varying levels of andrographolide addition using Fura-2AM dye by cell-based fluorescent microscopic analysis
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Reversible antagonist activity at recombinant human TAS2R8 assessed as recovery of suppression of andrographolide-induced intracellular calcium level at 1 uM preincubated for 60 secs followed by andrographolide addition measured after 5 mins washout period using Fura-2AM dye by cell-based fluorescent microscopic analysis
Antagonist activity at recombinant human TAS2R8 assessed as inhibition of andrographolide-induced intracellular calcium level at 1 uM preincubated for 60 secs followed by andrographolide addition using Fura-2AM dye by cell-based fluorescent microscopic analysis
Antagonist activity at recombinant human TAS2R8 stably expressed in cells co-expressing Galpha16gust44 assessed as inhibition of andrographolide-induced intracellular calcium level measured for 100 secs by fluo-4 dye based FLIPR assay
Assay data:55 Active, 42 Activity ≤ 1 µM, 56 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Antagonist activity at recombinant human TAS2R8 stably expressed in cells co-expressing Galpha16gust44 assessed as inhibition of andrographolide-induced intracellular calcium level at 10 uM measured for 100 secs by fluo-4 dye based FLIPR assay relative to control
Assay data:1 Tested
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
SummaryPubMed Citation
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
SummaryRelated BioAssays by Depositor
Identification of proteins required for the processing of the EWS-FLI1 nascent transcript
Assay data:59220 Tested
InfectX: Pathogen infection siRNA screens
Assay data:115372 Tested
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