Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Inhibition of UCHL3 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human his tagged UCH-L3 by Westernblot analysis
Assay data:1 Tested
Inhibition of UCHL3 (unknown origin) using Ub-AMC as substrate at 0.1 uM preincubated for 60 mins followed by substrate addition by fluorescence assay relative to control
Inhibition of UCHL3 (unknown origin) assessed as reduction in RAD51 deubiquitination incubated for 4 hrs
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of UCHL3 (unknown origin)
Inhibition of recombinant human UCHL3 expressed in baculovirus infected Sf9 cells using ubiquitin-AMC as substrate preincubated for 30 mins followed by substrate addition by fluorescence spectrophotometric assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 3 Tested
Inhibition of UCHL3 (unknown origin) using ubiquitin-AMC as substrate by fluorescence assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of UCHL3 (unknown origin) incubated for 20 mins by fluorescence assay
Inhibition of UCHL3 (unknown origin) using Ub-AMC as substrate preincubated for 30 mins followed by substrate addition by fluorescence assay
Inhibition of UCHL3 in human HEK293 cells using HA-Ub-VME as substrate at 0.1 to 10 uM incubated for 1 hr by immunoblotting analysis
Assay data:2 Active, 2 Tested
Inhibition of GST-tagged recombinant UCHL3 (unknown origin) using Ub-Lys-TAMRA as substrate incubated for 30 mins by fluorescence polarization assay
Assay data:2 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Enzyme Inhibition Assay from Article 10.1016/j.bmc.2007.07.016: "Identification of novel chemical inhibitors for ubiquitin C-terminal hydrolase-L3 by virtual screening."
Assay data:8 Tested
Fluorometric Ub-AMC Hydrolysis Assay from Article 10.1021/acschembio.7b00543: "Cell Lysate-Based AlphaLISA Deubiquitinase Assay Platform for Identification of Small Molecule Inhibitors."
Assay data:4 Active, 7 Tested
UCHL3 inhibition assay with Ub-Rho-morpholine as substrate
SummaryRelated BioAssays by Target
Inhibition of recombinant human UCHL3 assessed as reduction in cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ub-Rho 110 as substrate incubated for 30 mins by fluorescence based assay
Inhibition of human GST-tagged UCHL3 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis
Assay data:3 Tested
Inhibition of UCHL3 in HEK293T cell lysates assessed as enzyme recovery at 250 uM using HA-tagged vinyl-sulfone as substrate preincubated for 40 mins followed by 10-fold dilution and subsequent substrate addition measured within 15 mins by Western blot analysis
Inhibition of UCHL3 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis
Inhibition of UCHL3 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis
Inhibition of human recombinant UCHL3 assessed as reduction in cleavage of luminescent substrate Ub-AML at 100 uM
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on