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IC50 Assay from Article 10.1111/cbdd.12469: "Enzymatic Studies of Isoflavonoids as Selective and Potent Inhibitors of Human Leukocyte 5-Lipo-Oxygenase."
Assay data:20 Active, 15 Activity ≤ 1 µM, 26 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
IC50 Assay from Article 10.1111/cbdd.12157: "In vitro study of isoflavones and isoflavans as potent inhibitors of human 12- and 15-lipoxygenases."
Assay data:4 Active, 1 Activity ≤ 1 µM, 17 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of human epithelial cell 15-LOX-2
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human 15-LOX-2 expressed in HEK293T cells assessed as a reduction in 15-HETE production using arachidonic acid as substrate incubated for 20 mins
Assay data:3 Active, 2 Activity ≤ 1 µM, 4 Tested
Substrate activity at human 15-LOX-2 assessed as chemical reaction by RP-HPLC analysis
Assay data:3 Tested
Pseudo-peroxidase activity in human 15-LOX-2 assessed as redox activity of the compound by measuring degradation of 13-HpODE at 20 uM by UV/Vis pseudo-peroxidase assay
Inhibition of mouse 15-LOX2 assessed as enzymatic rate using arachidonic acid as substrate by UV/Vis spectrophotometric analysis
Uncompetitive inhibition of human 15-LOX-2 assessed as equilibrium dissociation constant from secondary site by measuring 15-HpETE by Dixon plots and Dixon replots analysis
Assay data:4 Active, 5 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Competitive inhibition of human 15-LOX-2 assessed as equilibrium dissociation constant from catalytic site by measuring 15-HpETE by Dixon plots and Dixon replots analysis
Assay data:5 Active, 4 Activity ≤ 1 µM, 5 Tested
Inhibition of human 15-LOX-2 assessed as enzymatic rate using arachidonic acid as substrate by UV/Vis spectrophotometric analysis
Assay data:5 Active, 3 Activity ≤ 1 µM, 5 Tested
Inhibition of human recombinant N-terminal His-tagged 15-LOX2 expressed in Escherichia coli assessed as residual activity at 42 uM using arachidonic acid as substrate preincubated for 5 mins followed by substrate addition measured after 20 mins in dark by ferric ion oxidation-xylenol orange assay relative to control
Assay data:30 Tested
Inhibition of human recombinant N-terminal His-tagged 15-LOX2 expressed in Escherichia coli using arachidonic acid as substrate preincubated for 5 mins followed by substrate addition measured after 20 mins in dark by ferric ion oxidation-xylenol orange assay
Human 15-LOX-2 (Lipoxygenases)
Assay data:1 Active, 1 Tested
Binding affinity to recombinant human 15-Lipoxygenase-2 at 10 uM
Inhibition of N-terminal His6-tagged human 15-LOX-2 using arachidonic acid as substrate by UV-visible spectrophotometric analysis
Inhibition of human 15-LOX-2 expressed in Sf9 cells assessed as reduction in conversion of arachidonic acid to 15-HETE pre-incubated 5 min before arachidonic acid substrate addition by HPLC-UV spectroscopy
Inhibition of human reticulocyte 15-lipoxygenase-2 by UV-visible spectrophotometry
Assay data:4 Tested
Inhibition of N-terminal His6-tagged human epithelial 15-lipoxygenase-2 using arachidonic acid as substrate by UV-vis spectrometric analysis
Inhibition of N-terminal His6-tagged human epithelial 15-LOX2 using arachidonic acid as substrate by UV-vis spectrophotometric analysis
Inhibition of N-terminal His6-tagged human prostate epithelial 15-LOX2 pseudoperoxidase activity assessed as reduction of active ferric ion to inactive ferrous state measured decomposition of 13-(S)-HPODE at 40 uM after 30 mins by iron-xylenol orange staining assay
Assay data:6 Tested
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