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pHi change assay with SLC9A2
Assay data:1 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of rat NHE2
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of rabbit NHE2
Assay data:2 Active, 2 Activity ≤ 1 µM, 3 Tested
Inhibition of NHE2 (unknown origin)
Assay data:5 Active, 3 Activity ≤ 1 µM, 7 Tested
Inhibition of NHE-2 (unknown origin)
Inhibition of rabbit NHE-2
Inhibition of rat NHE-2
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of human NHE2 expressed in chinese hamster LAP1 cells assessed as reduction in pHi recovery measured for 400 secs by BACEF-AM dye based fluorescence analysis
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
Inhibition of rabbit NHE2 expressed in chinese hamster LAP1 cells assessed as reduction in pHi recovery measured for 400 secs by BACEF-AM dye based fluorescence analysis
Assay data:1 Tested
SummaryRelated BioAssays by Target
Inhibition of human NHE2 expressed in CHO-K1 cells assessed as inhibition of Na+/Ca+ exchange activity measured after 5 mins by BACEF-AM dye based fluorescence analysis
Inhibition of human NHE2 expressed in chinese hamster AP1 cells preincubated for 7.5 mins followed by addition of NaCl measured after 5 mins by BACEF-AM dye based fluorescence analysis
Assay data:2 Active, 3 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of human NHE2 expressed in LAP1 cell assessed as intracellular pH recovery measured for 2 mins by CECF-AM dye based FLIPR assay
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human NHE2 expressed in Ap1 cell line
Assay data:1 Active, 3 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Sodium/hydrogen exchanger antiport activity was measured using [22]Na+ uptake inhibition assay in acidified rabbit erythrocytes
Assay data:38 Active, 36 Activity ≤ 1 µM, 38 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of 5-N-(ethyl isopropyl)-amiloride[EIPA]-sensitive Na+ uptake into acidified rabbit erythrocytes.
Assay data:113 Active, 109 Activity ≤ 1 µM, 113 Tested
In vitro concentration of compound required to inhibit NHE-2 mediated intracellular maximal pH recovery by 50%
Assay data:2 Active, 7 Tested
Compound is screened in AP1 cells expressing human NHE-2 for sodium hydrogen exchange activity
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