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Binding affinity of Yersinia enterocolitica YopH assessed as protein stabilization by measuring change in melting temperature by thermal denaturation assay (Rvb = 36.8 +/- 0.2 degreeC)
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity of Yersinia enterocolitica YopH assessed as structural changes in protein by measuring emission spectra by fluorescence assay
Non-competitive mixed inhibition of Yersinia enterocolitica YopH assessed as inhibition constant for enzyme-substrate complex
Non-competitive mixed inhibition of Yersinia enterocolitica YopH assessed as inhibition constant
Potency index, ratio of chicoric-acid IC50 to test compound IC50 for inhibition of Yersinia enterocolitica YopH
Inhibition of recombinant Yersinia enterocolitica YopH assessed as reduction in conversion of pNPP to nitrophenol and inorganic phosphate using pNPP as substrate by microplate reader assay
Irreversible inhibition of Yersinia enterocolitica YopH assessed as enzyme recovery activity by dilution assay relative to control
Assay data:2 Tested
Non-competitive inhibition of Yersinia enterocolitica YopH assessed as inhibition constant using varying level of pNPP as substrate by Lineweaver-Burk plot analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of Yersinia enterocolitica YopH
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of recombinant Yersinia enterocolitica YopH using p-nitrophenyl phosphate as substrate preincubated for 10 mins followed by substrate addition and measured every minute for 10 mins by UV-VIS spectrophotometric analysis
Assay data:4 Active, 4 Tested
Experimentally measured binding affinity data (Ki) for protein-ligand complexes derived from PDB
Assay data:3598 Active, 421 Activity ≤ 1 nM, 2233 Activity ≤ 1 µM, 3598 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Inhibition of Yersinia enterocolitica recombinant YopH using pNPP as substrate after 10 mins by spectrophotometry
Assay data:2 Active, 12 Tested
Inhibition of Yersinia enterocolitica recombinant YopH using pNPP as substrate at 25 uM after 10 mins by spectrophotometry
Assay data:12 Tested
Competitive inhibition of Yersinia enterocolitica recombinant YopH using pNPP as substrate after 10 mins by Line-weaver Burk plot analysis
Assay data:3 Active, 5 Tested
Noncompetitive inhibition of Yersinia enterocolitica recombinant YopH using pNPP as substrate after 10 mins by Line-weaver Burk plot analysis
Inhibition of Yersinia pseudotuberculosis phosphatase activity of secreted YopH
Assay data:3 Tested
Inhibition of Yersinia pseudotuberculosis phosphatase activity of secreted YopH at 100 uM
Assay data:4 Tested
Inhibition of Yersinia pseudotuberculosis phosphatase activity of secreted YopH at 50 uM
Inhibition of Yersinia pseudotuberculosis phosphatase activity of secreted YopH at 20 uM
Inhibition of Yersinia pseudotuberculosis phosphatase activity of secreted YopH at 10 uM
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